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Test Code SPECI Specimen Source Identification

Reporting Name

Specimen Source Identification

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Determining specimen origin when the patient identity of a specimen is in question

Method Name

Polymerase Chain Reaction (PCR)/Microsatellite Markers

Advisory Information

Chain of custody documentation is not available. This test is not intended for medico-legal or forensic purposes.

Shipping Instructions

Specimen preferred to arrive within 96 hours of collection.

Necessary Information

1. Due to the complex nature of this test, direct communication (eg, pathologist to pathologist) is required to avoid delays in sample processing and ensure an understanding of relevant case details.

2. A brief letter that includes the following 4 pieces of information is required for all orders:

a. Reason for testing, including detailed information regarding what specific comparisons are requested.

b. Clear identification of the known and unknown specimens.

c. Copies of all existing pathology reports pertaining to submitted issue specimens.

d. Contact information for the ordering physician.

Specimen Required

Patient Preparation: A previous bone marrow transplant from an allogenic donor will interfere with testing. Call 800-533-1710 for instructions for testing patients who have received a bone marrow transplant.


Specimen Type: Whole blood


Preferred: Lavender top (EDTA) or yellow top (ACD)

Acceptable: Any anticoagulant

Specimen Volume: 3 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Send specimen in original tube.

Specimen Stability Information: Ambient (preferred)/Refrigerated


Specimen Type: Tissue block or slide

Collection Instructions:

1. Submit formalin-fixed, paraffin-embedded (FFPE) tissue block (preferred) or 4 to 10 unstained sections (each 5-microns thick) plus 1 slide stained with hematoxylin and eosin.

2. The number of unstained sections required depends on the amount of tissue that can be used for analysis.

3. For very small tissue fragments, 10 sections are recommended; for large tissue fragments, 4 sections are generally sufficient.

4. If known and unknown specimens are within the same block, include labeled hematoxylin-and-eosin slide identifying the known and unknown specimens.

5. Specimen ID tests involving very small fragments of tissue, including most floaters, are performed at the discretion of the reviewing pathologist. Cases involving floaters are usually rejected due to an insufficient amount of the floater tissue.

Specimen Type


Specimen Minimum Volume

Blood: 0.5 mL
Tissue: see Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Varies

Reject Due To

All specimens will be evaluated by Mayo Clinic Laboratories for test suitability.


An interpretive report will be provided.

Day(s) and Time(s) Performed

Monday, Wednesday; 10 a.m.

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

81265-Comparative analysis using Short Tandem Repeat (STR) markers; patient and comparative specimen (eg, pre-transplant recipient and donor germline testing, post-transplant non-hematopoietic recipient germline [eg, buccal swab or other germline tissue sample] and donor testing, twin zygosity testing or maternal cell contamination of fetal cells


Added as needed:

81266 each additional specimen (eg additional cord blood donor, additional fetal samples from different cultures, or additional zygosity in multiple birth pregnancies)


LOINC Code Information

Test ID Test Order Name Order LOINC Value
SPECI Specimen Source Identification 81147-1


Result ID Test Result Name Result LOINC Value
53350 Result 81147-1
53351 Interpretation 69047-9
53352 Reason for Referral 42349-1
53353 Specimen 31208-2
53354 Source 31208-2
54450 Tissue ID 80398-1
52942 Method 49549-9
53355 Released By 18771-6

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
_STR1 Comp Analysis using STR (Bill only) No No
_STR2 Add'l comp analysis w/STR (Bill Only) No No