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HelpTest Code Billings Clinic: 4804 Mayo: CTDC Connective Tissue Diseases Cascade, Serum
Reporting Name
Connective Tissue Disease Cascade,SPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Useful For
Evaluation of patients with signs and symptoms compatible with connective tissue diseases
Initial evaluation of patients in clinical situations in which the prevalence of disease is low (6)
This test is not recommended for:
-Testing in clinical situations in which there is a high prevalence of connective tissue diseases (eg, rheumatology specialty practice)
-Follow-up evaluation of patients with known connective tissue diseases
Method Name
Enzyme-Linked Immunosorbent Assay (ELISA)
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Information: Centrifuge and aliquot serum into plastic vial.
Specimen Type
SerumSpecimen Minimum Volume
0.7 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 21 days | |
| Frozen | 21 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | OK |
Reference Values
ANTINUCLEAR ANTIBODIES (ANA)
≤1.0 U (negative)
1.1-2.9 U (weakly positive)
3.0-5.9 U (positive)
≥6.0 U (strongly positive)
Reference values apply to all ages.
CYCLIC CITRULLINATED PEPTIDE ANTIBODIES, IgG
<20.0 U (negative)
20.0-39.9 U (weak positive)
40.0-59.9 U (positive)
≥60.0 U (strong positive)
Reference values apply to all ages.
Interpretation
Interpretive comments are provided.
See individual test IDs for additional information.
Differential testing for Ro52 and Ro60 antibodies in SS-A/Ro positive patients may be useful in the diagnosis of specific CTD clinical subset, disease stratification, and prognosis. Consider testing for Ro52 and Ro60 antibodies (ROPAN / Ro52 and Ro60 Antibodies, IgG, Serum) if the patient is positive for SS-A/Ro.
Day(s) Performed
Monday through Saturday
Report Available
3 to 4 daysTest Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86038
86200
83516-Centromere (if appropriate)
83516-Ribosome (if appropriate)
86225-ds-DNA AB IgG, Serum (if appropriate)
86235 x 6-RNP, Sm, SS-B, SS-A, Jo 1, and Scl 70 (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| CTDC | Connective Tissue Disease Cascade,S | 95267-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| ANA2 | Antinuclear Ab, S | 94875-2 |
| CCP | Cyclic Citrullinated Peptide Ab, S | 33935-8 |
| IM_01 | Interpretation | 69048-7 |
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| ANA2 | Antinuclear Ab, S | Yes | Yes |
| CCP | Cyclic Citrullinated Peptide Ab, S | Yes | Yes |
| IM_01 | Interpretation | No | Yes |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| CMA | Centromere Ab, IgG, S | Yes | No |
| CASMT | ANA2 Cascade | No | No |
| RIB | Ribosome P Ab, IgG, S | Yes | No |
| ENAE | Ab to Extractable Nuclear Ag Eval,S | Yes | No |
| ADNA1 | dsDNA Ab, IgG, S | Yes | No |
Testing Algorithm
If antinuclear antibodies are greater than or equal to 3.0 U, then antibodies to double-stranded DNA (dsDNA), extractable nuclear antigen evaluation, ribosome P, and centromere are performed at an additional charge.
For more information see Connective Tissue Disease Cascade.