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Test Code Billings Clinic: 7072 Chlamydia trachomatis and Neisseria gonorrhoeae Detection by RNA Amplification

Performing Laboratory

Billings Clinic Laboratory: Microbiology

Methodology

Nucleic Acid Amplification (GEN-PROBE)

Specimen Requirements

Swab specimen must be collected using an APTIMA Collection Multitest Swab. These swabs are contained in the APTIMA Collection Kit.

 

Specimen Type: Vaginal

Container/Tube: APTIMA Collection Multitest Swab

Specimen Volume: Swab

Collection Instructions:

  1. Insert swab 2 in into the opening of the vagina, and rotate swab gently for 30 seconds. Make sure swab touches vaginal wall to absorb moisture by the swab.
  2. Place second swab into transport tube provided in collection kit. Snap off swab at score line so swab fits into closed tube.
  3. Cap tube securely, and label tube with patient's entire name, and date and time of collection.
  4. Transport and store swab container at 2 to 30° C (refrigerate is preferred temperature).  Swab is stable at 2 to 30° C for 60 days. If longer storage is needed, freeze at -20 to -70 degrees C for 12 months.

Specimen Type: Meatal (Males Only)

Container/Tube: APTIMA Collection Multitest Swab

Specimen Volume: Swab

Collection Instructions:

  1. Roll the swab at the tip or outside the opening of the penis.
  2. Uncircumcised penis will have to roll the forskin down before starting collection.
  3. Place swab in transport tube provided in collection kit. Snap off swab at score line so swab fits into closed tube.
  4. Cap tube securely, and label tube with patient's entire name, and date and time of collection.
  5. Transport and store swab container at 2 to 30° C (refrigerate is preferred temperature).  Swab is stable at 2 to 30° C for 60 days. If longer storage is needed, freeze at -20 to -70 degrees C for 12 months.

 

Specimen Type: Extragenital (throat and rectal)

Container/Tube: APTIMA Collection Multitest Swab

Specimen Volume: Swab

Collection Instructions:

  1. Swab site using Aptima Collection Unisex Swab (T583).  (The white swab provided within the collection kit is a cleaning swab and should not be used for collection. Discard the white cleaning swab.)
  2. Place swab in transport tube provided in collection kit. Snap off swab at score line so swab fits into closed tube.
  3. Cap tube securely, and label tube with patient's entire name, and date and time of collection.
  4. Transport and store swab container at 4°C to 30°C, or -20°C and -70°C until tested.
  5. Specimens must be assayed with the Aptima Combo 2 Assay within 60 days of collection.

 

Specimen Type: Vaginal (Self-collection)

Please see "Vaginal Self-Collection Guide" under "Resources"

Reference Values

  • Chlamydia trachomatis RNA and Neisseria gonorrhoeae RNA not detected and presumed negative for Chlamydia trachomatis and Neisseria gonorrhoeae by RNA amplification
  • All positive results will be called to the City/County Health Nurse as per Montana state law.

Day(s) Test Set Up

Monday through Friday

Test Classification and CPT Coding

87492 - Infectious agent detection by nucleic acid (DNA or RNA); Chlamydia trachomatis, quantification
87592 - Infectious agent detection by nucleic acid (DNA or RNA); Neisseria gonorrhoeae, quantification

LOINC Code Information

See individual tests