Test Code Billings Clinic: 7072 Chlamydia trachomatis and Neisseria gonorrhoeae Detection by RNA Amplification
Performing Laboratory
Billings Clinic Laboratory: Microbiology
Methodology
Nucleic Acid Amplification (GEN-PROBE)
Specimen Requirements
Swab specimen must be collected using an APTIMA Collection Unisex Swab. These swabs are contained in the APTIMA Collection Kit.
Specimen Type: Endocervix
Container/Tube: APTIMA Collection Unisex Swab
Specimen Volume: Swab
Collection Instructions:
- Use cleaning swab (white shaft) to remove excess mucus from endocervix and discard.
- Insert second swab (blue shaft) 1 to 1.5 cm into endocervical canal, and rotate swab gently for 30 seconds. Avoid touching vaginal wall when removing swab.
- Place second swab into transport tube provided in collection kit. Snap off swab at score line so swab fits into closed tube.
- Cap tube securely, and label tube with patient's entire name, and date and time of collection.
- Transport and store swab container at 2 to 30° C (refrigerate is preferred temperature). Swab is stable at 2 to 30° C for 60 days. If longer storage is needed, freeze at -20 to -70 degrees C for 12 months.
Specimen Type: Urethra (Males Only)
Container/Tube: APTIMA Collection Unisex Swab
Specimen Volume: Swab
Collection Instructions:
- Patient should not have urinated for at least 1 hour prior to collection.
- With a rotating movement, insert swab (blue shaft) 2 to 4 cm into urethra.
- Once inserted, rotate swab gently at least 1 full rotation using sufficient pressure to ensure swab comes into contact with all urethral surfaces. Allow swab to remain inserted for 2 to 3 seconds.
- Place swab in transport tube provided in collection kit. Snap off swab at score line so swab fits into closed tube.
- Cap tube securely, and label tube with patient's entire name, and date and time of collection.
- Transport and store swab container at 2 to 30° C (refrigerate is preferred temperature). Swab is stable at 2 to 30° C for 60 days. If longer storage is needed, freeze at -20 to -70 degrees C for 12 months.
Specimen Type: Extragenital (throat and rectal)
Container/Tube: APTIMA Collection Unisex Swab
Specimen Volume: Swab
Collection Instructions:
- Swab site using Aptima Collection Unisex Swab (T583). (The white swab provided within the collection kit is a cleaning swab and should not be used for collection. Discard the white cleaning swab.)
- Place swab in transport tube provided in collection kit. Snap off swab at score line so swab fits into closed tube.
- Cap tube securely, and label tube with patient's entire name, and date and time of collection.
- Transport and store swab container at 4°C to 30°C, or -20°C and -70°C until tested.
- Specimens must be assayed with the Aptima Combo 2 Assay within 60 days of collection.
Specimen Type: Vaginal (Self-collection)
Please see "Vaginal Self-Collection Guide" under "Resources"
Reference Values
- Chlamydia trachomatis RNA and Neisseria gonorrhoeae RNA not detected and presumed negative for Chlamydia trachomatis and Neisseria gonorrhoeae by RNA amplification
- All positive results will be called to the City/County Health Nurse as per Montana state law.
Day(s) Test Set Up
Monday through Friday
Test Classification and CPT Coding
87492 - Infectious agent detection by nucleic acid (DNA or RNA);
Chlamydia trachomatis, quantification
87592 - Infectious agent detection by nucleic acid (DNA or RNA);
Neisseria gonorrhoeae, quantification
LOINC Code Information
See individual tests