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HelpTest Code Billings Clinic:7441 Mayo:EOSDF Chronic Eosinophilia, Diagnostic FISH, Varies
Ordering Guidance
This test is intended to be ordered when the entire chronic eosinophilia fluorescence in situ hybridization (FISH) panel is needed.
If limited chronic eosinophilia FISH probes are preferred, order EOSMF / Chronic Eosinophilia, Specified FISH, Varies.
At follow-up, targeted chronic eosinophilia probes can be evaluated based on the abnormalities identified in the diagnostic study. Order EOSMF/ Chronic Eosinophilia, Specified FISH, Varies. and request a specific probe to evaluate the known genomic abnormality.
Paraffin embedded tissue testing is not available for these probe sets.
Necessary Information
A reason for testing and a flow cytometry and/or a bone marrow pathology report should be submitted with each specimen. The laboratory will not reject testing if this information is not provided however, appropriate testing and interpretation may be compromised or delayed. If not provided, an appropriate indication for testing may be entered by Mayo Clinic Laboratories.
Specimen Required
Submit only 1 of the following specimens:
Preferred
Specimen Type: Bone marrow
Container/Tube:
Preferred: Yellow top (ACD)
Acceptable: Green top (heparin) or lavender top (EDTA)
Specimen Volume: 2-3 mL
Collection Instructions:
1. It is preferable to send the first aspirate from the bone marrow collection.
2. Invert several times to mix bone marrow.
3. Send bone marrow specimen in original tube. Do not aliquot.
Acceptable
Specimen Type: Blood
Container/Tube:
Preferred: Yellow top (ACD)
Acceptable: Green top (heparin) or lavender top (EDTA)
Specimen Volume: 6 mL
Collection Instructions:
1. Invert several times to mix blood.
2. Send whole blood specimen in original tube. Do not aliquot.
Useful For
Detecting a neoplastic clone associated with the common chromosome abnormalities seen in patients with myeloid/lymphoid neoplasms with eosinophilia and gene rearrangement (including PDGFRA, PDGFRB, FGFR1, JAK2, and ABL1).
Supporting the diagnosis of malignancy if a clone is present
An adjunct to conventional chromosome studies.
Testing Algorithm
This test includes a charge for the probe application, analysis, and professional interpretation of results for 5 probe sets (11 individual fluorescence in situ hybridization [FISH] probes). Additional charges will be incurred for all reflex or additional probe sets performed.
The panel includes testing for the following kinase activating chromosome abnormalities, using the following FISH probes:
4q12 deletion or rearrangement, FIP1L1, CHIC2 ,PDGFRA
5q32 rearrangement, PDGFRB
8p11.2 rearrangement, FGFR1
9p24.1 rearrangement, JAK2
9q34 rearrangement, ABL1
Appropriate ancillary probes may be performed at consultant discretion to render comprehensive assessment. Any additional probes will have the results included within the final report and will be performed at an additional charge.
In the absence of a CHIC2 deletion, if an extra or atypical CHIC2 or PDGFRA signal is identified, reflex testing will be performed using the PDGFRA break-apart probe set to evaluate for the presence or absence of a PDGFRA rearrangement.
If a PDGFRB rearrangement is identified, reflex testing using the PDGFRB/ETV6 probe set will be performed to evaluate for the presence or absence of t(5;12)(q32;p13) -PDGFRB::ETV6 fusion.
If an ABL1 rearrangement is identified, reflex testing using the ABL1/BCR probe set will be performed to evaluate for the presence or absence of t(9;22)(q34;q11.2) - ABL1::BCR fusion.
Method Name
Fluorescence In Situ Hybridization (FISH)
Reporting Name
Chronic Eosinophilia, Diag FISHSpecimen Type
VariesSpecimen Minimum Volume
Blood: 2 mL
Bone Marrow: 1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Ambient (preferred) | ||
| Refrigerated | |||
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Reference Values
An interpretive report will be provided.
Interpretation
A neoplastic clone is detected when the percent of cells with an abnormality exceeds the normal reference range for any given probe.
The absence of an abnormal clone does not rule out the presence of a neoplastic disorder.
Day(s) Performed
Monday through Friday
Report Available
7 to 10 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
88271 x11, 88275 x5, 88291 x1-FISH Probe, Analysis, Interpretation; 5 probe sets
88271 x2, 88275 x1–FISH Probe, Analysis; each additional probe set (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| EOSDF | Chronic Eosinophilia, Diag FISH | In Process |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 609588 | Result Summary | 50397-9 |
| 609589 | Interpretation | 69965-2 |
| 609590 | Result Table | 93356-4 |
| 609591 | Result | 62356-1 |
| GC080 | Reason for Referral | 42349-1 |
| GC081 | Specimen | 31208-2 |
| 609592 | Source | 31208-2 |
| 609593 | Method | 85069-3 |
| 609594 | Additional Information | 48767-8 |
| 609595 | Disclaimer | 62364-5 |
| 609596 | Released By | 18771-6 |
Forms
If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.