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Test Code DCORT 11-Deoxycortisol, Serum

Reporting Name

11-Deoxycortisol, S

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Diagnostic workup of patients with congenital adrenal hyperplasia


Part of metyrapone testing in the workup of suspected secondary or tertiary adrenal insufficiency


Part of metyrapone testing in the differential diagnostic workup of Cushing syndrome

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Specimen Type


Necessary Information

Indicate if specimen was drawn before or after metyrapone.

Specimen Required


Preferred: Red top

Acceptable: Serum gel

Specimen Volume: 0.5 mL

Collection Instructions: Morning (8 a.m.) specimen is preferred.

Specimen Minimum Volume

0.4 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 28 days
  Ambient  28 days
  Frozen  28 days

Reject Due To

Gross hemolysis Reject
Gross lipemia OK
Gross icterus OK

Reference Values

≤18 years: <344 ng/dL

>18 years: 10-79 ng/dL


For SI unit Reference Values, see


In a patient suspected of having congenital adrenal hyperplasia (CAH), elevated serum 11-deoxycortisol levels indicate possible 11 beta-hydroxylase deficiency. However, not all patients will show baseline elevations in serum 11-deoxycortisol levels. In a significant proportion of cases, increases in 11-deoxycortisol levels are only apparent after adrenocorticotropic hormone (ACTH)(1-24) stimulation.(1)


Serum 11-deoxycortisol levels below 1,700 ng/dL 8 hours after metyrapone administration is indicative of probable adrenal insufficiency. The test cannot reliably distinguish between primary and secondary or tertiary causes of adrenal failure, as neither patients with pituitary failure, nor those with primary adrenocortical failure, tend to show an increase of 11-deoxycortisol levels after metyrapone is administered.


See Steroid Pathways in Special Instructions.

Day(s) and Time(s) Performed

Tuesday; 10 a.m.

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
DCORT 11-Deoxycortisol, S 1657-6


Result ID Test Result Name Result LOINC Value
46923 11-Deoxycortisol, S 1657-6

Testing Algorithm

See Steroid Pathways in Special Instructions.

Special Instructions