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HelpTest Code KITVS KIT Asp816Val Mutation Analysis, Varies
Reporting Name
KIT Asp816Val Mutation Analysis, VPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Useful For
Diagnosing systemic mastocytosis using blood or bone marrow specimens
Method Name
Allele-Specific Oligonucleotide Polymerase Chain Reaction (PCR)
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Whole blood
Container/Tube: Lavender top (EDTA) or yellow top (ACD)
Specimen Volume: 3 mL
Collections Instructions:
1. Invert several times to mix blood.
2. Send specimen in original tube. Do not aliquot.
3. Label specimen as blood.
Specimen Stability Information: Ambient (preferred) 7 days/Refrigerate 7 days
Specimen Type: Bone marrow
Container/Tube: Lavender top (EDTA) or yellow top (ACD)
Specimen Volume: 2 mL
Collections Instructions:
1. Invert several times to mix bone marrow.
2. Send specimens in original tube. Do not aliquot.
3. Label specimen as bone marrow.
Specimen Stability Information: Ambient (preferred) 7 days/Refrigerate 7 days
Specimen Type: Extracted DNA from blood or bone marrow
Container/Tube: 1.5- to 2-mL tube
Specimen Volume: Entire specimen
Collection Instructions:
1. Label specimen as extracted DNA from blood or bone marrow.
2. Provide indication of volume and concentration of DNA.
Specimen Stability Information: Frozen (preferred)/Refrigerated/Ambient
Specimen Type
VariesSpecimen Minimum Volume
Blood, Bone Marrow: 1 mL
Extracted DNA: 50 mcL at 20 ng/mcL concentration
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Varies | ||
Reject Due To
| Gross hemolysis | Reject |
| Moderately to severely clotted Bone marrow biopsies Paraffin-embedded bone marrow clots Paraffin-embedded tissue Slides Paraffin shavings |
Reject |
Reference Values
An interpretive report will be provided indicating the mutation status as positive or negative.
Interpretation
The test will be interpreted as positive or negative for KIT Asp816Val.
Day(s) Performed
Monday through Friday
Report Available
4 to 7 daysTest Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
81273
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| KITVS | KIT Asp816Val Mutation Analysis, V | 55201-8 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| MP055 | Specimen Type | 31208-2 |
| 607982 | Interpretation | 69047-9 |
| 607983 | Signing Pathologist | 19139-5 |
Forms
1. Hematopathology Patient Information (T676)
2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726)) with the specimen.