Test Code ALDEF Alpha Defensin, Lateral Flow Assay, Synovial Fluid
Reporting Name
Alpha Defensin, Synovial Fluid, LFAPerforming Laboratory
Mayo Clinic Laboratories in RochesterUseful For
Detection of alpha defensins 1-3, human host response proteins, in synovial fluid of adults with a total joint replacement who are being evaluated for revision surgery
This test is not intended to be used to determine timing for reimplantation in 2-stage procedures.
Method Name
Lateral Flow Assay (LFA)
Ordering Guidance
This test should only be used for patients with a total joint prosthesis.
Necessary Information
Specimen source and type of joint are required. If not obtained from a prosthetic joint, testing will be canceled.
If ordering electronically, answers must be provided for the order entry questions.
If not ordering electronically, specimen source and type of joint must be provided on the request form.
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Plain red-top tube
Submission Container/Tube: Plastic vial
Specimen Volume: 0.6 mL
Specimen Type
Synovial FluidSpecimen Minimum Volume
0.10 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Synovial Fluid | Refrigerated | 7 days |
Reject Due To
Gross hemolysis | Reject |
Reference Values
Negative
Reference values apply to all ages.
Interpretation
Negative:
No alpha defensin detected in synovial fluid, suggesting absence of prosthetic joint infection.
Positive:
Alpha defensin in synovial fluid detected suggesting presence of prosthetic joint infection. Additional microbiologic studies (eg, culture, molecular detection) are recommended.
Day(s) Performed
Monday through Friday, Sunday
Report Available
Same day/1 dayTest Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
83518
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
ALDEF | Alpha Defensin, Synovial Fluid, LFA | 94718-4 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
607711 | Alpha Defensin, Synovial Fl, LFA | 94718-4 |
JOINT | Is this for a prosthetic joint? | 86955-2 |
ADSRC | Alpha Defensin Source | 72923-6 |