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HelpTest Code AMPIP Amyloid Protein Identification, Paraffin, Mass Spectrometry
Reporting Name
Amyloid Protein ID, Par, LC MS/MSPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Useful For
Definitive identification of amyloid proteins
Method Name
Histological Stain/Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Ordering Guidance
This test should only be ordered on patients for whom a primary diagnosis has already been established. If a patient does not have a primary diagnosis, order PATHC / Pathology Consultation or refer to the Pathology Consultation Ordering Algorithm.
If a pathology consultation is desired in addition to this test, order PATHC / Pathology Consultation alone and send the required paperwork with specimen. Indicate that amyloid protein identification is desired. If needed, this test will be added by the reviewing pathologist and will be reported with the consultation. For more information see PATHC / Pathology Consultation.
Shipping Instructions
Attach the green pathology address label included in the kit to the outside of the transport container.
Necessary Information
1. Preliminary pathology report and history are required.
2. Include performed Congo red slide
3. A brief explanatory note or consultative letter is also recommended.
Specimen Required
Specimen Type: Formalin-fixed or B5-fixed paraffin-embedded tissue block
Collection Instructions:
1. Do not send fixed tissue slides for testing. Testing can only be done on paraffin-embedded tissue blocks.
2. If Congo red stain has already been performed, send Congo red stained slide along with the tissue block.
Specimen Type
AMYLOIDSpecimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| AMYLOID | Ambient (preferred) | ||
| Refrigerated | |||
Reject Due To
| Fixed tissue slides Wet/frozen tissue Cytological smears Nonformalin fixed tissue Nonparaffin embedded tissue |
Reject |
Reference Values
An interpretive report will be provided.
Interpretation
An interpretation will be provided.
Day(s) Performed
Monday through Friday
Report Available
7 to 15 daysTest Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
88313
82542 (if appropriate)
88380 (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| AMPIP | Amyloid Protein ID, Par, LC MS/MS | 101405-9 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 71185 | Interpretation | 50595-8 |
| 71186 | Participated in the Interpretation | No LOINC Needed |
| 71187 | Report electronically signed by | 19139-5 |
| 71189 | Material Received | 81178-6 |
| 71592 | Disclaimer | 62364-5 |
| 72109 | Case Number | 80398-1 |
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-Cardiovascular Test Request (T724)
-Hematopathology/Cytogenetics Test Request (T726)
-Renal Diagnostics Test Request (T830)
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| MLCPC | Microdissection, Laser Capture | No, (Bill Only) | No |
| MSPTC | Mass Spectrometry | No, (Bill Only) | No |
Testing Algorithm
In all cases with adequate tissue, an initial Congo red stain is performed before mass spectrometry testing to confirm positivity, and the pattern of amyloid deposition can be considered when interpreting mass spectrometry results.
In some instances, per pathologist discretion, a different initial Congo red stain may be performed using SS2PC / Special Stain, Group II, Other (Bill Only).
-If the stain is negative for amyloid, then this test will not be performed, and only the SS2PC will be charged.
-If the stain is positive for amyloid, this test will be performed, and the SS2PC billing charge will be credited.
A pathology consultation is typically not required. If the amyloid subtyping results do not fit the clinical findings, PATHC / Pathology Consultation may be added if appropriate, upon client approval.
For more information see Amyloidosis: Laboratory Approach to Diagnosis.