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Test Code BARTB Bartonella, Molecular Detection, PCR, Blood

Reporting Name

Bartonella PCR, B

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Aiding in the diagnosis of Bartonella infection when Bartonella DNA would be expected to be present in blood, especially endocarditis

Method Name

Real-Time Polymerase Chain Reaction (PCR)


Ordering Guidance


If this test result is negative and there is a strong suspicion of disease caused by these organisms, consider BART / Bartonella Antibody Panel, IgG and IgM, Serum and Warthin-Starry tissue stain (PATHC / Pathology Consultation) testing.



Specimen Required


The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by Bartonella species DNA is unlikely.

 

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Royal blue top (EDTA), pink top (EDTA), or sterile vial containing EDTA-derived aliquot

Specimen Volume: 1 mL

Collection Instructions: Send specimen in original tube (preferred).


Specimen Type

Whole Blood EDTA

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood EDTA Refrigerated (preferred) 7 days
  Ambient  7 days
  Frozen  7 days

Reject Due To

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Reference Values

Not applicable

Interpretation

A positive result indicates the presence of Bartonella species DNA.

 

A negative result indicates the absence of detectable Bartonella DNA but does not negate the presence of the organism and may occur due to inhibition of PCR, sequence variability underlying primers or probes, or the presence of Bartonella DNA in quantities less than the limit of detection of the assay.

Day(s) Performed

Monday through Friday

Report Available

2 to 7 days

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87801

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BARTB Bartonella PCR, B 16275-0

 

Result ID Test Result Name Result LOINC Value
SRC98 Specimen Source 31208-2
56056 Bartonella PCR 16275-0

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.