Home
HelpTest Code BETV2 BET v2 (Profilin), IgE, Serum
Reporting Name
BET v2 (Profilin), IgE, SPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Useful For
Evaluation of patients suspected birch pollen allergy
Evaluation of patients with suspected peanut allergy
Evaluation of patients with oral allergy syndrome to other pollens or plant-based foods
Method Name
Fluorescence Enzyme Immunoassay (FEIA)
Ordering Guidance
For a listing of allergens available for testing, see Allergens - Immunoglobulin E (IgE) Antibodies.
BET v2 is also available as part of the PEANT / Peanut, IgE with Reflex to Peanut Components, IgE, Serum. This reflex test includes Ara h2, h1, h3, h6, h9, and Bet v2 (profilin) if the initial Peanut IgE test is detectable.
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL for every 5 allergens requested
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Type
SerumSpecimen Minimum Volume
For 1 allergen: 0.3 mL
For more than 1 allergen: (0.05 mL x number of allergens) + 0.25 mL deadspace
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 14 days | |
| Frozen | 90 days | ||
| Ambient | 7 days |
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Reference Values
|
Class |
IgE kU/L |
Interpretation |
|
0 |
<0.10 |
Negative |
|
0/1 |
0.10-0.34 |
Borderline/Equivocal |
|
1 |
0.35-0.69 |
Equivocal |
|
2 |
0.70-3.49 |
Positive |
|
3 |
3.50-17.4 |
Positive |
|
4 |
17.5-49.9 |
Strongly positive |
|
5 |
50.0-99.9 |
Strongly positive |
|
6 |
≥100 |
Strongly positive |
Concentrations ≥0.70 kU/L (Class 2 and above) will flag as abnormally high.
Interpretation
Profilins are potentially cross-reactive allergenic proteins found in many plant pollens and tissues. IgE antibodies to the profilin Bet v2, while associated with birch pollen sensitivity, also represent a minor peanut allergen marker as it is cross-reactive with the peanut profilin Ara h5. The presence of antibodies to profilin Bet v2 is typically associated with milder allergic reactions and oral allergy syndrome.
Day(s) Performed
Monday through Friday
Report Available
Same day/1 to 3 daysTest Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86008
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| BETV2 | BET v2 (Profilin), IgE, S | 30985-6 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| BETV2 | BET v2 (Profilin), IgE, S | 30985-6 |
Forms
If not ordering electronically, complete, print, and send an Allergen Test Request (T236) with the specimen.