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HelpTest Code Billings Clinic:1417 Mayo:AMBF Amylase, Body Fluid
Reporting Name
Amylase, BFUseful For
Evaluation of patients with a pathological accumulation of fluid to determine whether pancreatic inflammation, pancreatic fistula, or esophageal rupture may be contributing
Aiding in the diagnosis of pancreatitis
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Body FluidOrdering Guidance
For pancreatic cyst fluid specimens, order AMLPC / Amylase, Pancreatic Cyst Fluid. Testing will be changed to AMLPC if this test is ordered on that specimen type.
Necessary Information
1. Date and time of collection are required.
2. Specimen source is required.
Specimen Required
Specimen Type: Body fluid
Preferred Source:
-Peritoneal fluid (peritoneal, abdominal, ascites, paracentesis)
-Pleural fluid (pleural, chest, thoracentesis)
-Drain fluid (drainage, Jackson Pratt [JP] drain)
-Pericardial fluid
Acceptable Source: Write in source name with source location (if appropriate)
Collection Container/Tube: Sterile container
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions:
1. Centrifuge to remove any cellular material and transfer into a plastic vial.
2. Indicate the specimen source and source location on label.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Body Fluid | Refrigerated (preferred) | 7 days | |
| Frozen | 30 days | ||
| Ambient | 24 hours |
Reference Values
An interpretive report will be provided
Day(s) Performed
Monday through Sunday
Test Classification
This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
82150
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| AMBF | Amylase, BF | 1795-4 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| AMBFL | Amylase, BF | 1795-4 |
| FLD21 | Fluid Type, Amylase | 14725-6 |
Interpretation
Peritoneal and drain fluid amylase activity in non-pancreatic peritoneal fluid is often less than or equal to the serum amylase activity. Ascites associated with pancreatitis typically has amylase activity at least 5-fold greater than serum.(1)
Normal pleural fluid amylase activity is typically less than the upper limit of normal serum amylase and has a ratio of pleural fluid amylase to serum amylase ratio less than 1.0.(3)
All Other Fluids: Body fluid amylase activity may become elevated due to the presence of pancreatitis, esophageal rupture, or amylase producing neoplasms. Results should be interpreted in conjunction with serum amylase and other clinical findings.
Report Available
Same day/1 to 2 daysReject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
| Anticoagulant or additive, breast milk, nasal secretions, gastric secretions, bronchoalveolar lavage (BAL or bronchial washings), feces, colostomy/ ostomy, saliva, sputum, urine, vitreous fluid, or pancreatic cyst | Reject |
Method Name
Enzymatic Colorimetric
Forms
If not ordering electronically, complete, print, and send a Gastroenterology and Hepatology Test Request (T728) with the specimen.