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Test Code Billings Clinic: 1919 Quest: 91691 HIV-1 RNA, Quantitative, Real-Time PCR with Reflex to Genotype (RTI, PI, Integrase)

Performing Laboratory

Quest Diagnostics Infectious Disease

Useful For

HIV-1 RNA, Quantitative, Real-Time PCR with Reflex to Genotype (RTI, PI, Integrase) - Monitor viral load in HIV-1 patients undergoing antiretroviral therapy and automatically reflex to drug resistance testing for PR, RT and Integrase drugs in patients failing to achieve or maintain virologic suppression.

Methodology

Real-Time Polymerase Chain Reaction

Specimen Requirements

Preferred Specimen(s):

7 mL plasma collected in an EDTA (lavender-top) tube

 

Minimum Volume

3.7 mL

 

Collection Instructions

  • Do not freeze whole blood or any samples stored in primary tube. 
  • Freshly drawn specimens (whole blood) may be stored at 2-25° C for up to 24 hours prior to centrifugation.
  • Separate plasma from cells within 24 hours of collection by centrifugation.

 

Tranport Temperature

Frozen

Storage/Stability

Specimen Type Temperature Time
EDTA Plasma Refrigerated 6 days
  Frozen (preferred) 30 days

Reject Due To

  • Serum
  • Heparinzed plasma
  • Specimens sent at ambient temperature
  • Non-centrifuged specimens

Reference Values

Reference Range(s)

HIV-1 RNA, QN, PCR Not detected copies/mL
HIV-1 RNA, QN, PCR Not detected Log copies/mL

Day(s) Test Set Up

Set up: Mon-Sat

Report Available: 2-4 days

Test Classification and CPT Coding

87536 - Infectious agent detection by nucleic acid (DNA or RNA); HIV-1, quantification, includes reverse transcription when performed

87900 (if appropriate) - Infectious agent drug susceptibility phenotype prediction using regularly updated genotypic bioinformatics

87901 (if appropriate) - Infectious agent genotype analysis by nucleic acid (DNA or RNA); HIV-1, reverse transcriptase and protease regions

87906 (if appropriate) - Infectious agent genotype analysis by nucleic acid (DNA or RNA); HIV-1, other region (eg, integrase, fusion)

LOINC Code Information

20447-9     HIV 1 RNA

Profile/Testing Information

If HIV-1 RNA, Quantitative, Real-Time PCR viral load is >400 copies/mL, HIV-1 Genotype and HIV-1 Integrase Genotype will be performed at an additional charge