Sign in →

Test Code Billings Clinic: 1920 Quest: 34949 HIV-1 Genotype

Performing Laboratory

Quest Diagnostics Infectious Disease

Useful For

HIV-1 Genotype - This test is intended for use in conjunction with clinical presentation and other laboratory markers of disease progress for the clinical management of HIV-1 infected patients. The test identifies drug resistance-associated mutations in the HIV-1 protease and reverse transcriptase genes. It can be used to predict antiretroviral drug resistance before initiation of therapy and in patients experiencing virologic failure while on therapy.

Methodology

DNA Sequencing • Reverse Transcriptase Polymerase Chain Reaction (RT-PCR)

Specimen Requirements

Preferred Specimen(s):

2 mL plasma collected in an EDTA (lavender-top) tube

 

Minimum Volume

0.6 mL

 

Collection Instructions

  • Freshly drawn specimens (whole blood) may be stored at 2° C to 25° C for up to 24 hours prior to centrifugation.
  • Separate plasma from cells within 24 hours of collection by centrifugation. 

 

Tranport Temperature

Frozen

Storage/Stability

Specimen Type Temperature Time
EDTA Plasma Refrigerated 6 days
  Frozen (preferred) 42 days
  Ambient 24 hours

Reject Due To

  • Serum
  • Specimens sent frozen in the original collection tube
  • Whole blood >24 hours old
  • Frozen whole blood

Reference Values

See Laboratory Report
 

Day(s) Test Set Up

Set up: Daily

Report Available: 4 - 9 days

Test Classification and CPT Coding

87900 - Infectious agent drug susceptibility phenotype prediction using regularly updated genotypic bioinformatics

87901 - Infectious agent genotype analysis by nucleic acid (DNA or RNA); HIV-1, reverse transcriptase and protease regions

LOINC Code Information

48558-1     HIV Genotype

Profile/Testing Information

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.