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Test Code Billings Clinic: 3215 IPF (Immature Platelet Fraction)

Performing Laboratory

Billings Clinic Laboratory: Hematology

Useful For

  • In the evaluation of thrombocytopenia, quantification of immature platelets may be used as an index of bone marrow thrombopoietic activity, analogous to the red cell reticulocyte count in the evaluation of anemia. 
  • This test may be useful in the differential diagnosis of thrombocytopenia.  In general, an increased IPF (% and #) may be supportive of peripheral sequestration/utilization such as that seen in ITP or TTP and other conditions in which platelet production is increased; a normal IPF in the face of thrombocytopenia may indicate a production problem such as that seen in marrow failure syndromes and myelodysplasia. 
  • This test is not intended to be diagnostic of any condition but merely serves to provide additional information that mat be useful.

Methodology

Fluorescent: Flow Cytometry method is used to identify and isolate the immature platelets from the mature platelets based on size and the RNA and DNA content. The immature platelet fraction (IPF) is expressed as a proportional value of the total fluorescent platelet count.

Specimen Requirements

  • Specimen must arrive within 48 hours of draw (refrigerated).
  • Draw a full, lavender-top (EDTA) tube
  • Send 4 mL of unprocessed EDTA whole blood promptly
  • Immediately after draw invert several times to mix blood
  • Do not centrifuge

Storage/Stability

Specimen Type Temperature Time
EDTA Whole Blood Refrigerated 48 hours
  Ambient 24 hours

Reject Due To

  • Age of specimen > 48 hours
  • Clotted specimen
  • Gross hemolysis

Reference Values

IPF%:

1.2 - 8.6 %


IPF, Absolute:

3.6 - 20.0 x 103 / uL

 

Day(s) Test Set Up

Daily

Test Classification and CPT Coding

85055 - Reticulated platelet assay

 

LOINC Code Information

Reporting Name LOINC Code
IPF % 71693-6
IPF Abs 71692-8