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Test Code Billings Clinic: 4360 Tobramycin, Peak, Plasma or Serum

Performing Laboratory

Billings Clinic Laboratory: Chemistry

Useful For

Monitoring adequacy of serum concentration during tobramycin therapy

Methodology

Particle-enhance turbidimetric inhibition immunoassay (PETINIA)

Specimen Requirements

Submit only 1 of the following specimens:

Serum or plasma should be physically separated from cells as soon as possible with a maximum limit of two hours from the time of collection.

 

Plasma

  • Draw blood in a green-top (lithium heparin) tube(s).
  • Spin down and send 1.0 mL of heparinized plasma frozen.
  • Plasma for a peak level should be drawn 30 to 45 minutes after completion of infusion of an intravenous dose.
  • Date and time of last dose are required on request form for processing.

Serum

  • Draw blood in a plain, red-top tube(s) or a serum gel tube(s).
  • Spin down and send 1.0 mL of serum frozen
  • Plasma for a peak level should be drawn 30 to 45 minutes after completion of infusion of an intravenous dose.
  • Date and time of last dose are required on request form for processing.

Storage/Stability

Specimen Type Temperature Time
Plasma, Serum Refrigerated 3 days
  Frozen (preferred) 1 month
  Ambient 8 hours

 

Reject Due To

  • Specimens stored at room temperature > 8 hours
  • Hemolysis

Reference Values

Cystic Fibrosis: 20 - 35 ug/mL

Traditional Dosing: 8 - 12 ug/mL

Extended Interval Dosing: 20 - 25 ug/mL

Day(s) Test Set Up

Daily

Test Classification and CPT Coding

80200 - Tobramycin

LOINC Code Information

Reporting Name LOINC Code
Tobramycin, Peak 4057-6
Tobra Dose Date/Time 45285-4