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Test Code Billings Clinic: 4391 Estradiol, Serum

Important Note

Results from patients taking biotin supplements or receiving high-dose biotin therapy should be interpreted with caution due to possible interference with this test.

 

The drug Fulvestrant (FASLODEX) may cause falsely elevated estradiol results in immunoassays.  For patients being treated with Fulvestrant, an alternate method that is not expected to show cross-reactivity to Fulvestrant, such as liquid chromatography-mass spectrometry (LC-MS), should be used.

Performing Laboratory

Billings Clinic Laboratory: Chemistry

Useful For

  • Monitoring estradiol levels is important in evaluating amenorrhea, precocious puberty, the onset of menopause and infertility in men and women. 
  • Monitoring estradiol levels is essential during in vitro fertilization, because the timing of recovery of oocytes depends on follicular development, which in turn depends on the estradiol level.

Methodology

Chemiluminescence

Specimen Requirements

Serum should be physically separated from cells as soon as possible with a maximum limit of two hours from the time of collection.

Serum

  • Draw blood in a plain, red-top tube(s) or a serum gel tube(s).
  • Spin down and send 1.0 mL of serum frozen

Storage/Stability

Specimen Type Temperature Time
Serum Refrigerated 2 days
  Frozen (preferred) 6 months
  Ambient 20 hours

 

Reject Due To

  • Samples frozen/thawed more than once
  • Gross hemolysis

Reference Values

Male:                                                         0.0 - 39.8 pg/mL

Female:

Menstruating

Follicular:                                              19.5 - 144.2 pg/mL

Mid-cycle:                                              63.9 - 356.7 pg/mL

Luteal:                                                    55.8 - 214.2 pg/mL

Post-Menopausal

Untreated:                                                0.0 - 32.2 pg/mL

Day(s) Test Set Up

Daily

Test Classification and CPT Coding

82670 - Estradiol

LOINC Code Information

Reporting Name LOINC Code
Estradiol 2243-4