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Test Code Billings Clinic: 6846 Mayo: MTRNA Trichomonas vaginalis, Nucleic Acid Amplification, Varies

Reporting Name

T.vaginalis, Misc, Amplified RNA

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Detecting Trichomonas vaginalis in prostatic massage (VBIII) fluid or male urethral swabs

Method Name

Transcription-Mediated Amplification


Necessary Information


Specimen source is required.



Specimen Required


This test should only be performed in men.

 

Submit only 1 of the following specimens:

 

Specimen Type: Post-prostatic massage fluid (VBIII)

Supplies: Aptima Urine Transport Tube (T582)

Container/Tube: Aptima Urine Specimen Transport Tube

Specimen Volume: 15 to 20 mL

Collection Instructions:

1. Patient should not have urinated for at least 1 hour prior to specimen collection.

2. Patient should void a small amount of urine prior to prostatic massage. Pre-massage urine can be discarded or submitted for other testing as applicable.

3. Patient then ceases voiding and a prostatic massage is performed by the urologist or other health care professional.

4. Collect post-massage fluid into a sterile, plastic, preservative-free container.

5. Transfer 2 mL of post-massage fluid specimen into the Aptima urine specimen transport tube using the disposable pipette provided within 24 hours of collection. The correct volume of fluid has been added when the fluid level is between the black fill lines on the Aptima urine transport tube.

 

Specimen Type: Urethral (male only)

Supplies: Aptima Unisex Swab Collection Kit (T583)

Container/Tube: Aptima Unisex Swab

Specimen Volume: Swab

Collection Instructions:

1. Urethral specimens must be collected using an Aptima Unisex Swab Collection kit.

2. Patient should not have urinated for at least 1 hour prior to collection.

3. With a rotating movement, insert swab (blue shaft) 2 to 4 cm into urethra.

4. Once inserted, rotate swab gently at least 1 full rotation using sufficient pressure to ensure swab comes into contact with all urethral surfaces. Allow swab to remain inserted for 2 to 3 seconds.

5. Place swab in the Aptima transport tube provided in collection kit. Snap off swab at score line so swab fits into closed tube.

6. Cap tube securely and label tube with patient's entire name and collection date and time.


Specimen Type

Varies

Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 30 days APTIMA VIAL
  Ambient  30 days APTIMA VIAL

Reject Due To

Transport tubes containing a cleaning swab or more than 1 swab Reject

Reference Values

Negative

Interpretation

A positive result indicates the presence of nucleic acid from Trichomonas vaginalis and is strongly supportive of a diagnosis of trichomoniasis.

 

A negative result indicates the absence of nucleic acid from T vaginalis.

 

A negative result does not exclude the possibility of infection. If clinical indications strongly suggest gonococcal or chlamydial infection, additional specimens should be collected for testing.

 

A result of inconclusive indicates that a new specimen should be collected.

 

The predictive value of an assay depends on the prevalence of the disease in any specific population. In settings with a high prevalence of sexually transmitted infections, positive assay results have a high likelihood of being true-positive results. In settings with a low prevalence of sexually transmitted infections or in any setting in which a patient's clinical signs and symptoms or risk factors are inconsistent with infection, positive results should be carefully assessed, and the patient retested by other methods if appropriate.

Day(s) Performed

Monday through Sunday

Report Available

1 to 4 days

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87661

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MTRNA T.vaginalis, Misc, Amplified RNA 46154-1

 

Result ID Test Result Name Result LOINC Value
SRC6 SOURCE: 31208-2
35034 T.vaginalis, Misc, amplified RNA 46154-1

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.