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Test Code Billings Clinic: 6858 Neisseria gonorrhoeae, ThinPrep® Liquid Pap Vial

Performing Laboratory

Billings Clinic Laboratory: Microbiology

Methodology

Nucleic Acid Amplification (GenProbe)

 

This test is to be used only in conjunction with Cytology, Cervical/Vaginal, Liquid Prep, ThinPrep®. To order Neisseria gonorrhoeae Detection by RNA Amplification using GenProbe order Billings Clinic:  #7069 “Neisseria gonorrhoeae Detection by RNA Probe Amplification.”

Specimen Requirements

Collect specimen using PreservCyt® ThinPrep® Vial including Wallach Papette® (broom-like device) as follows:

 

Patient Preparation: 

  1. Optimal time for collection of Pap smear is 2 weeks after first day of last menstrual period.
  2. Patient should be instructed not to use vaginal medications, spermicides, or douches 48 hours prior to collection of smear.
  3. Patient should also refrain from intercourse 24 hours prior to collection of smear.

Preparation of the Cervix:

  1. Warm water may be used to lubricate device.
  2. Lubricant jelly should be avoided as it often obscures cellular material making cytologic evaluation difficult.
  3. Excess blood, mucus, or inflammatory material should be removed with a cotton swab prior to taking sample.

Materials and Reagents:

  1. The Wallach Papette® (broom-like device) used to collect specimen
  2. ThinPrep® Preservative Fluid Collection vial.

Storage Requirements:

  1. Storage condition for ThinPrep® Preservative Fluid without cytologic specimens is up to 36 months from date of manufacture at ambient temperature (15-30° C.)
  2. Storage requirements for ThinPrep® Preservative Fluid with cytologic specimens is 6 weeks at ambient temperature (15-30° C.)

Specimen Collection:

  1. Contact cervix with the Wallach Papette® (broom-like device) and insert central bristles into cervical canal deep enough to allow shorter bristles to fully contact ectocervix.
  2. While maintaining gentle pressure in direction of cervix, rotate brush 5 times in a clockwise direction.
  3. Rinse the broom in PreservCyt® as soon as possible by pushing broom to bottom of the vial 10 times forcing bristles to separate and swirling the broom vigorously to further remove material. 
  4. Discard the collection device. 
  5. Place cap on vial, making sure cap is tightened to avoid leakage.
  6. Label transport tube with patient’s name (first and last), date and actual time of collection, and type of specimen.
  7. Send specimen ambient or refrigerated at 2° C to 8° C.
  8. Specimen source is required on request form for processing.

 

Reject Due To

  • Swab specimen not in PreservCyt® ThinPrep® 2000TM Specimen Collection System and Transport Kit.
  • Specimen collected with large-tipped cleaning swab.
  • Moderately bloody or grossly mucoid specimen.
  • Spermicidal agents and feminine powder sprays interfere with assay and should not be used prior to collection.

Reference Values

Neisseria gonorrhoeae RNA not detected and presumed negative for Neisseria gonorrhoeae.

Automatic call-back:  any positive
 

All positive results will be called to the City/County Health Nurse as per Montana state law.

Day(s) Test Set Up

Monday through Friday

Test Classification and CPT Coding

87592

LOINC Code Information

Reporting Name LOINC Code
N. gonorrhoeae (Cervix) 50388-8