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Test Code Billings Clinic: 8318 HIV Type 1 and Type 2 Antigen/Antibodies (Combined Assay), EDTA Plasma

Important Note

The performance of this assay has not been established with neonatal specimens.

Performing Laboratory

Billings Clinic Laboratory: Chemistry

Useful For

  • Screening for HIV-1 and/or HIV-2 infection in asymptomatic atients
  • Diagnosis of HIV-1 and/or HIV-2 infection in symptomatic patients
  • Follow-up testing of individuals with reactive results from rapid HIV tests

Methodology

Sandwich Immunoassay

Specimen Requirements

EDTA plasma should be physically separated from cells as soon as possible with a maximum limit of two hours from the time of collection.

 

EDTA Plasma

  • Draw blood in Purple-top tube
  • Spin down and send 1 mL of EDTA plasma refrigerated.
  • Label tube as EDTA Plasma
  • If there is a delay in transport of ≥6 days, send specimen frozen in plastic vial.

Storage/Stability

Specimen Type Temperature Time
EDTA Refrigerated 14 days
  Frozen (preferred) 8 months
  Ambient 24 hours

Reject Due To

  • Heat-inactivated specimens
  • Gross Hemolysis

Reference Values

Negative (reported as nonreactive)
 

Automatic call-back:  all positives

Positive results are confirmed and differentiated between HIV-1 and HIV-2

Day(s) Test Set Up

Daily

Test Classification and CPT Coding

86703 - HIV-1 and HIV-2, single result

 

LCD or NCD test: ICD-10 code is required for this test. When appropriate, obtain a properly executed ABN and submit the ABN with test order(s). See “Medical Necessity and Advanced Beneficiary Notice (ABN) Policy and Form” under Resources for a copy of a form and additional information.

LOINC Code Information

Reporting Name LOINC Code
HIV 1 & 2 Ag/Ab 48345-3

 

Profile/Testing Information

Repeat testing is performed if reactive. If repeat is reactive, test will reflex to:

HIV Confirmation, State

 

This test may automatically reflex to other tests. An additional fee may be added. The ordering physician/provider must indicate if the reflex test is NOT DESIRED.

 

Indicate on order if no reflex test is desired.