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Test Code Billings Clinic: 8571 Mayo: FACTV Coagulation Factor V Activity Assay, Plasma

Reporting Name

Coag Factor V Assay, P

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Diagnosing congenital deficiencies (rare) of coagulation factor V

 

Evaluating acquired deficiencies associated with liver disease, factor V inhibitors, myeloproliferative disorders, and intravascular coagulation and fibrinolysis

 

Investigation of prolonged prothrombin time or activated partial thromboplastin time

Method Name

Optical Clot-Based


Ordering Guidance


Coagulation testing is highly complex, often requiring the performance of multiple assays and correlation with clinical information. For that reason, consider ordering a Coagulation Consultation.



Necessary Information


If priority specimen, mark request form, give reason, and request a call-back.



Specimen Required


Patient Preparation: Patient must not be receiving coumadin (warfarin) or heparin therapy. (If not possible for medical reasons, note on request.)

Collection Container/Tube: Light-blue top (3.2% sodium citrate)

Specimen Type: Platelet-poor plasma

Collection Container/Tube: Light-blue top (3.2% sodium citrate)

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. Specimen must be collected prior to factor replacement therapy.

2. For complete instructions, see Coagulation Guidelines for Specimen Handling and Processing

3. Centrifuge, transfer all plasma into a plastic vial, and centrifuge plasma again.

4. Aliquot plasma into a plastic vial, leaving 0.25 mL in the bottom of centrifuged vial.

5. Freeze plasma immediately (no longer than 4 hours after collection) at -20° C or, ideally,  -40° C or below.

Additional Information:

1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.

2. Each coagulation assay requested should have its own vial.


Specimen Type

Plasma Na Cit

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma Na Cit Frozen 14 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject

Reference Values

>1 month: 70%-165%

<1 month: Normal, full-term and premature newborn infants may have mildly decreased levels (≥30% to 35%) which reach adult levels within 21 days postnatal.

 

*See Pediatric Hemostasis References section in Coagulation Guidelines for Specimen Handling and Processing

Interpretation

Acquired deficiencies are much more common than congenital.

 

Patients that are congenitally deficient homozygous generally have activity levels less than or equal to10% to 20%.

 

Patients that are congenitally deficient heterozygous generally have activity levels less than or equal to 50%.

 

Congenital deficiency may occur in combined association with factor VIII deficiency.

Day(s) Performed

Monday through Saturday

Report Available

1 to 2 days

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

85220

LOINC Code Information

Test ID Test Order Name Order LOINC Value
FACTV Coag Factor V Assay, P 3193-0

 

Result ID Test Result Name Result LOINC Value
FACTV Coag Factor V Assay, P 3193-0