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Test Code Billings Clinic: 9499 MYD88 Mutation Analysis

Performing Laboratory

NeoGenomics Laboratories

Useful For

This test is designed to detect the point mutation c.794T>, p.L265P in the MYD88 gene.

 

MYD88 mutation is the most common genetic abnormality in the activated B-cell-like (ABC) subtype of diffuse large B-cell lymphoma (DLBCL), detected in 40% of cases. Mutations are rare in the germinal center B-cell-like (GCB) subtype, so mutation analysis can be useful to differentiate between the ABC and GCB subtypes. The L265P mutation is present in >90% of Waldenstrom's macroglobulinemia (WM) and has been associated with increased risk of progression to WM in IgM MGUS patients. MYD88 is also implicated in susceptibility to BTK inhibitors in the treatment of B-cell neoplasms.

 

Methodology

Polymerase Chain Reaction (PCR)

Specimen Requirements

Specimen Types:

  • Peripheral blood: 5 mL in EDTA tube.
  • Bone marrow: 2 -3 mL in EDTA tube.
  • FFPE tissue: Paraffin block is preferred. Alternatively, send 1 H&E slide plus 5-10 unstained slides cut at 5 or more microns. Please use positively-charged slides and 10% NBF fixative. Do not use zinc fixatives.
  • Fresh tissue: 0.5 - 1 cm3 in RPMI

 

Processing and Transport:

  • Do not centrifuge
  • Send sample refrigerated. Use a refrigerated cold pack for transport.
  • Make sure cold pack is not in direct contact with the specimen.
  • DO NOT FREEZE

Reference Values

An interpretive report will be provided.

Day(s) Test Set Up

Monday - Friday, by 3 pm

TAT - 7 days

Test Classification and CPT Coding

81305

 

LOINC Code Information

Reporting Name LOINC Code
MYD88 Mutation Analysis In process