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HelpTest Code Billings Clinic: 9728 Mayo: LYWB Lyme Disease Antibody, Immunoblot, Serum
Reporting Name
Lyme Disease Ab, Immunoblot, SPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Useful For
Aiding in the diagnosis of systemic Lyme disease
This test should not be used as a screening assay.
Method Name
Immunoblot Microarray
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume:0.75 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Type
SerumSpecimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 14 days | |
| Frozen | 30 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
| Heat-inactivated specimen | Reject |
Reference Values
IgG: Negative
IgM: Negative
Reference values apply to all ages
Interpretation
Per Centers for Disease Control and Prevention criteria, the Lyme IgG Immunoblot is interpreted as positive if IgG-class antibodies are detected to greater than or equal to 5 Borrelia burgdorferi proteins, and the Lyme IgM Immunoblot is interpreted as positive if IgM-class antibodies are detected to greater than or equal to 2 B burgdorferi proteins. Immunoblot patterns not meeting these criteria should not be interpreted as positive. Epitopes from certain B burgdorferi proteins (eg, p41) are conserved across other bacteria, which may lead to the detection of IgM- and/or IgG-class antibodies on the Lyme disease immunoblots in patients without Lyme disease. Immunoblot should only be ordered on specimens that are positive or equivocal by a US Food and Drug Administration-licensed Lyme disease antibody screening test (eg, enzyme immunoassay). Results of the Lyme IgM immunoblot should not be considered in patients with greater than or equal to 30 days of symptoms.
|
Result |
Interpretation |
|
IgG and IgM negative |
Specific serologic response to B burgdorferi infection is not detected but cannot rule out early infection during which low or undetectable antibody levels to B burgdorferi may be present. If clinically indicated, a new serum specimen should be submitted in 7-14 days. |
|
IgG positive and IgM negative |
Consistent with infection with B burgdorferi at some time in the past |
|
IgG and IgM positive |
Consistent with active or previous infection for B burgdorferi. IgM blot criteria is of diagnostic utility only during the first 4 weeks of early Lyme disease. |
|
IgG negative and IgM positive |
IgM-class antibodies to B burgdorferi (Lyme disease) detected. Results are consistent with acute or recent infection with B burgdorferi. Testing of a new specimen collected in 7-14 days to demonstrate IgG seroconversion may be considered to confirm infection if the diagnosis is in doubt. IgM immunoblot results should only be considered as indicative of recent infection in patients presenting within 30 days of symptom onset. Consideration of IgM immunoblot results in patients with symptoms lasting >30 days is discouraged due to the risk of false positive IgM immunoblot results and/or prolonged IgM seropositivity following disease resolution. |
|
IgG and/or IgM uninterpretable |
Immunoblot invalid due to blurring or indistinct reactivity. Due to an invalid Lyme IgG immunoblot, an interpretation cannot be provided. Please submit a new specimen. |
Day(s) Performed
Monday, Wednesday, Friday
Report Available
Same day/1 to 4 daysTest Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86617 x 2
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| LYWB | Lyme Disease Ab, Immunoblot, S | 18203-0 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 5744 | IgG Immunoblot | 6320-6 |
| 2992 | IgG detected against: | 13502-0 |
| 23931 | IgM Immunoblot | 6321-4 |
| 23932 | IgM detected against: | 13503-8 |
| 6241 | Interpretation | 12781-1 |
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-General Request (T239)
Testing Algorithm
For information see Acute Tick-Borne Disease Testing Algorithm.