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HelpTest Code Billings Clinic: 9924 Mayo: HPFRP Helicobacter pylori with Clarithromycin Resistance Prediction, Molecular Detection, PCR, Feces
Reporting Name
H pylori + Clarithro Resist, PCR, FPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Useful For
Aiding in the diagnosis of Helicobacter pylori infection and prediction of clarithromycin resistance or susceptibility directly from stool
Method Name
Real-Time Polymerase Chain Reaction (PCR)
Ordering Guidance
Confirmation of eradication testing should not be ordered until 4 or more weeks after cessation of treatment.
Specimen Required
The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by Helicobacter pylori DNA is unlikely.
Patient Preparation:
1. For 4 weeks prior to testing, patient should not take antibiotics.
2. For 2 weeks prior to testing, patient should not take proton pump inhibitors or bismuth compounds.
Supplies: Culture and Sensitivity Stool Transport Vial (T058)
Specimen Type: Preserved feces
Submission Container/Tube: Commercially available transport system specific for recovery of enteric pathogens from fecal specimens (15 mL of nonnutritive transport medium containing phenol red as a pH indicator, either Cary-Blair or Para-Pak C and S)
Specimen Volume: 5 mL
Collection Instructions:
1. Collect fresh fecal specimen and submit 1 gram or 5 mL in container with transport medium.
2. Place feces in preservative within 2 hours of collection.
3. Place vial in a sealed plastic bag and send ambient or refrigerated. Specimens sent frozen will be rejected.
Specimen Type
FecalSpecimen Minimum Volume
1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Fecal | Ambient (preferred) | 7 days | |
| Refrigerated | 7 days |
Reject Due To
| Fecal swab ESwab transport medium Feces in gel transport medium ECOFIX preservative formalin PVA fixative Unpreserved stool |
Reject |
Reference Values
Not detected
Interpretation
A detected result indicates the presence of Helicobacter pylori 23S ribosomal RNA gene; also indicated is whether or not one the 3 most common 23S ribosomal RNA gene single nucleotide variations (A2143G, A2142G, and A2142C) associated with clarithromycin resistance is detected.
A not detected result for H pylori indicates the absence of detectable H pylori DNA but does not negate the presence of the organism and may occur due to inhibition of the polymerase chain reaction (PCR), sequence variability underlying primers or probes, or the presence of H pylori DNA in quantities less than the limit of detection of the assay.
Day(s) Performed
Monday, Wednesday, Friday
Report Available
4 to 6 daysTest Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
87798
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| HPFRP | H pylori + Clarithro Resist, PCR, F | 88509-5 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| HPSRC | Specimen Source | 31208-2 |
| 608002 | Helicobacter pylori Result | 91061-2 |
| 608003 | Clarithromycin Resistance Result | 88509-5 |
Forms
If not ordering electronically, complete, print, and send one of the following with the specimen:
1. Microbiology Test Request (T244)
Testing Algorithm
For more information see Helicobacter pylori Diagnostic Algorithm.