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Test Code CBSRP Coxiella burnetii (Q Fever), Molecular Detection, PCR, Serum

Reporting Name

Coxiella burnetii (Q fever) PCR, S

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Aiding in the diagnosis of Coxiella burnetii infection (ie, Q fever) using serum specimens

Method Name

Real-Time Polymerase Chain Reaction (PCR)


Specimen Required


The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by Coxiella burnetii DNA is unlikely.

 

Collection Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Submission Container/Tube: Sterile vial

Specimen Volume: 1 mL

Collection Instructions: Centrifuge and aliquot serum into a sterile vial within 2 hours of collection.


Specimen Type

Serum

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Frozen (preferred) 7 days
  Refrigerated  7 days

Reject Due To

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Reference Values

Not applicable

Interpretation

A positive result indicates the presence of Coxiella burnetii DNA.

 

A negative result indicates the absence of detectable C burnetii DNA but does not negate the presence of the organism and may occur due to inhibition of PCR, sequence variability underlying primers or probes, or the presence of C burnetii DNA in quantities less than the limit of detection of the assay.

Day(s) Performed

Monday through Friday

Report Available

2 to 7 days

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CBSRP Coxiella burnetii (Q fever) PCR, S 90443-3

 

Result ID Test Result Name Result LOINC Value
35189 Specimen Source 31208-2
35190 Coxiella burnetii PCR 90443-3

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.