Sign in →

Test Code DENGC Dengue Virus, Molecular Detection, PCR, Spinal Fluid

Reporting Name

Dengue Virus, PCR, CSF

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Aiding in the diagnosis of central nervous system infection caused by dengue virus

Method Name

Real-Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)


Ordering Guidance


The presence of dengue virus nucleic acid in cerebrospinal fluid or serum overlaps with the presence of dengue virus nonstructural protein 1 (NS1) antigen (DNSAG / Dengue Virus NS1 Antigen, Serum). Patients with a history of symptoms for more than 1 week may be negative by molecular tests (ie, real-time polymerase chain reaction) and may require serologic testing (DENVP / Dengue Virus Antibody/Antigen Panel, Serum) to confirm the diagnosis of dengue virus infection.



Specimen Required


Collection Container/Tube:

Preferred: Vial number 2

Acceptable: Any vial number

Submission Container/Tube: Sterile screw cap vial

Specimen Volume: 0.5 mL

Collection Instructions: Do not centrifuge or heat inactivate.


Specimen Type

CSF

Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time
CSF Refrigerated (preferred) 7 days
  Frozen  7 days

Reject Due To

Heat-inactivated specimen Reject

Reference Values

Negative

Reference values apply to all ages.

Interpretation

Positive:

The detection of dengue virus nucleic acid in cerebrospinal fluid (CSF) is consistent with acute-phase infection of the central nervous system.

Dengue virus nucleic acid may be detectable during the first 1 to 7 days following the onset of symptoms.

 

Negative:

The absence of dengue nucleic acid in CSF is consistent with the lack of acute-phase infection.

Dengue virus nucleic acid may not be detected if the CSF specimen is collected immediately following dengue virus infection (<24-48 hours) and is rarely detectable following 7 days of symptoms.

Day(s) Performed

Monday, Wednesday, Friday

Report Available

Same day/1 to 5 days

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
DENGC Dengue Virus, PCR, CSF 77958-7

 

Result ID Test Result Name Result LOINC Value
606371 Dengue Virus, PCR, CSF 77958-7

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244)with the specimen.