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HelpTest Code EOSMF Chronic Eosinophilia, Specified FISH, Varies
Ordering Guidance
This test is intended for instances when limited chronic eosinophilia fluorescence in situ hybridization (FISH) probes are needed. The FISH probes to be analyzed must be specified on the request, otherwise test processing may be delayed in order to determine the intended analysis. If specific probes are not included with this test request, this test may be canceled and automatically reordered by the laboratory as EOSDF / Chronic Eosinophilia, Diagnostic FISH, Varies.
If the entire chronic eosinophilia FISH panel is preferred, order EOSDF / Chronic Eosinophilia, Diagnostic, FISH, Varies.
Paraffin embedded tissue testing is not available for these probe sets.
This test is ordered for targeted FISH probes to be evaluated based on specific abnormalities or on abnormalities identified in the diagnostic sample.
At diagnosis, a EOSDF / Chronic Eosinophilia, Diagnostic, FISH, Varies panel and a conventional chromosome study should be performed.
Shipping Instructions
Advise Express Mail or equivalent if not on courier service.
Necessary Information
1. A list of probes requested for analysis is required. Probes available for this test are listed in the Testing Algorithm section.
2. A reason for testing should be with each specimen. The laboratory will not reject testing if this information is not provided; however, appropriate testing and interpretation may be compromised or delayed. If not provided, an appropriate indication for testing may be entered by Mayo Clinic Laboratories.
3. A pathology and/or flow cytometry report may be requested by the laboratory to optimize testing and aid in interpretation of results.
Specimen Required
Submit only 1 of the following specimens:
Preferred
Specimen Type: Bone marrow
Container/Tube:
Preferred: Yellow top (ACD)
Acceptable: Green top (heparin) or lavender top (EDTA)
Specimen Volume: 2-3 mL
Collection Instructions:
1. It is preferable to send the first aspirate from the bone marrow collection.
2. Invert several times to mix bone marrow.
3. Send bone marrow specimen in original tube. Do not aliquot.
Acceptable
Specimen Type: Blood
Container/Tube:
Preferred: Yellow top (ACD)
Acceptable: Green top (heparin) or lavender top (EDTA)
Specimen Volume: 6 mL
Collection Instructions:
1. Invert several times to mix blood.
2. Send whole blood specimen in original tube. Do not aliquot.
Useful For
Detecting a neoplastic clone associated with the common chromosome abnormalities seen in patients with myeloid/lymphoid neoplasms with eosinophilia and gene rearrangement (including PDGFRA, PDGFRB, FGFR1, JAK2, and ABL1) using specified probes set
Supporting a diagnosis of malignancy when a clone is present
An adjunct to conventional chromosome studies
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| EOSMB | Probe, Each Additional (EOSMF) | No, (Bill Only) | No |
| EOS3B | Probe, Tri-color (EOSMF) | No, (Bill Only) | No |
Testing Algorithm
This test includes a charge for the probe application, analysis, and professional interpretation of results for one probe set (2 individual fluorescence in situ hybridization [FISH] probes or 3 individual FISH probes). Additional charges will be incurred for all reflex or additional probe sets performed.
If the patient is being treated for known abnormalities, indicate the abnormality and which probes should be used.
When specified, any of the following probes will be performed:
4q12 deletion or rearrangement, FIP1L1, CHIC2, PDGFRA
4q12 rearrangement, PDGFRA
5q32 rearrangement, PDGFRB
t(5;12), PDGFRB/ETV6
8p11.2 rearrangement, FGFR1
9p24.1 rearrangement, JAK2
9q34 rearrangement, ABL1
t(9;22), BCR/ABL1
Appropriate ancillary probes may be performed at consultant discretion to render comprehensive assessment. Any additional probes will have the results included within the final report and will be performed at an additional charge.
For more information see Eosinophilia: Bone Marrow Diagnostic Algorithm.
Method Name
Fluorescence In Situ Hybridization (FISH)
Reporting Name
Chronic Eosinophilia, Spec FISHSpecimen Type
VariesSpecimen Minimum Volume
Blood: 2 mL
Bone Marrow: 1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Ambient (preferred) | ||
| Refrigerated | |||
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Reference Values
An interpretive report will be provided.
Interpretation
A neoplastic clone is detected when the percent of cells with an abnormality exceeds the normal reference range for any given probe.
The absence of an abnormal clone does not rule out the presence of a neoplastic disorder.
Day(s) Performed
Monday through Friday
Report Available
7 to 10 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
88271 x2, 88275 x1, 88291 x1-FISH Probe, Analysis, Interpretation; 1 probe sets
88271 x2, 88275 x1-FISH Probe, Analysis; each additional probe set (if appropriate)
88271 x1-FISH Probe; coverage for sets containing 3 probes (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| EOSMF | Chronic Eosinophilia, Spec FISH | In Process |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 614256 | Result Summary | 50397-9 |
| 614257 | Interpretation | 69965-2 |
| 614258 | Result Table | 93356-4 |
| 614259 | Result | 62356-1 |
| GC113 | Reason for Referral | 42349-1 |
| GC114 | Probes Requested | 78040-3 |
| GC115 | Specimen | 31208-2 |
| 614260 | Source | 31208-2 |
| 614261 | Method | 85069-3 |
| 614262 | Additional Information | 48767-8 |
| 614263 | Disclaimer | 62364-5 |
| 614264 | Released By | 18771-6 |
Special Instructions
Forms
If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.