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HelpTest Code GLICP CD8 T-Cell Immune Competence Panel, Global, Whole Blood
Reporting Name
CD8 Immune Competence PanelPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Useful For
Determining over immunosuppression within the CD8 T-cell compartment, when used on transplant recipients and patients with autoimmune disorders receiving therapy with immunosuppressant agents
Method Name
Flow Cytometry
Ordering Guidance
This test requires multiple whole blood specimens to perform all testing. If only one whole blood specimen type is received, only the testing associated with that specimen type will be performed. Be advised that this may change the degree of interpretation received with the report.
Shipping Instructions
Testing is performed Monday through Friday. Specimens not received by 4 p.m. (CST) on Friday may be canceled.
Collect and package specimens as close to shipping time as possible. Ship specimens overnight in an Ambient Shipping Box-Critical Specimens Only (T668) following the instructions in the mailer. It is recommended that specimens arrive within 24 hours of collection.
Samples arriving on the weekend and observed holidays may be canceled.
Necessary Information
Date and time of collection and ordering healthcare professional name and phone number are required.
Specimen Required
Two separate whole blood specimens, EDTA and (possibly 2 tubes) sodium heparin, are required.
For serial monitoring, it is recommended that specimens are collected at the same time of day.
Supplies: Ambient Shipping Box-Critical Specimens Only (T668)
Specimen Type: EDTA whole blood
Container/Tube: Lavender top (EDTA)
Specimen Volume: 3 mL
Collection Instructions:
1. Send whole blood specimen in original tube. Do not aliquot.
2. Label specimen as TBBS / Quantitative Lymphocyte Subsets: T, B, and Natural Killer (NK) Cells, Blood
Additional Information: For serial monitoring, it is recommended that specimen collection be performed at the same time of day.
Specimen Type: Sodium heparin whole blood
Container/Tube: Green top (sodium heparin)
Specimen Volume: 15 mL
Collection Instructions:
1. Send whole blood specimen in original tube. Do not aliquot.
2. Label specimen as GLIC / CD8 T-Cell Immune Competence, Global, Blood
Specimen Type
WB Sodium HeparinWhole Blood EDTA
Specimen Minimum Volume
Sodium heparin whole blood: 10 mL
EDTA whole blood: 1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| WB Sodium Heparin | Ambient | 48 hours | GREEN TOP/HEP |
| Whole Blood EDTA | Ambient | 52 hours | PURPLE OR PINK TOP/EDTA |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
Reference Values
The appropriate age-related reference values will be provided on the report.
Interpretation
Interferon-gamma (IFN-gamma) and CD107a and CD107b expression below the defined reference range are consistent with a global impairment in CD8 T-cell function, most likely due to over immunosuppression.
IFN-gamma, CD107a, and CD107b levels greater than the defined reference range are unlikely to have any clinical significance.
Day(s) Performed
Monday through Friday
Report Available
3 to 6 daysTest Classification
This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
86355-B cells, total count
86357-Natural killer (NK) cells, total count
86359-T cells, total count
86360-Absolute CD4/CD8 count with ratio
86356 x 2
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| GLICP | CD8 Immune Competence Panel | 80721-4 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 30643 | IFN-g | 95204-4 |
| 3321 | CD45 Total Lymph Count | 27071-0 |
| 30644 | CD107a/b | 95203-6 |
| 3316 | % CD3 (T Cells) | 8124-0 |
| 30645 | Interpretation | 69052-9 |
| 3322 | CD3 (T Cells) | 8122-4 |
| 3319 | % CD4 (T Cells) | 8123-2 |
| 3325 | CD4 (T Cells) | 24467-3 |
| 3320 | % CD8 (T Cells) | 8101-8 |
| 3326 | CD8 (T Cells) | 14135-8 |
| 3318 | % CD19 (B Cells) | 8117-4 |
| 3324 | CD19 (B Cells) | 8116-6 |
| 4054 | % CD16+CD56 (NK cells) | 8112-5 |
| 4055 | CD16+CD56 (NK cells) | 20402-4 |
| 3327 | 4/8 Ratio | 54218-3 |
| 6657 | Comment | 80722-2 |
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| TBBS | QN Lymphocyte Subsets: T, B, and NK | Yes | Yes |
| GLIC | CD8 Immune Competence, B | Yes | Yes |
Testing Algorithm
When multiple specimen types are required to perform a panel of tests, the laboratory will perform the tests for which the appropriate specimen type was received, and the laboratory will cancel those for which the appropriate specimen was not received.