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Test Code GSH Glutathione, Blood

Reporting Name

Glutathione, B

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Evaluation of neonatal hyperbilirubinemia, favism or chronic or episodic hemolysis or jaundice

 

Evaluation for gamma-glutamylcysteine synthetase deficiency

 

Evaluation for glutathione synthetase deficiency causing hemolytic anemia

 

Evaluation for generalized glutathione synthetase deficiency with 5-oxoprolinuria

Method Name

Kinetic Spectrophotometry (KS)


Specimen Required


Container/Tube:

Preferred: Yellow top (ACD solution B)

Specimen Volume: 6 mL

Collection Instructions: Send whole blood specimen in original tube. Do not transfer blood to other containers.


Specimen Type

Whole Blood ACD-B

Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood ACD-B Refrigerated 20 days

Reject Due To

Gross hemolysis Reject

Reference Values

≥12 months: 46.9-90.1 mg/dL RBC

Reference values have not been established for patients who are younger12 months of age.

Interpretation

Measurement of reduced glutathione (GSH) is used as a surrogate for the activity of the enzymes that contribute to normal levels of GSH within the red blood cell. GSH is associated with less than 25% of mean normal in individuals with deficiencies of gamma-glutamyl cysteine synthetase or glutathione synthetase.

 

Elevated concentrations of GSH are of uncertain significance. This finding can be nonspecific and is seen in normal neonates, pyrimidine-5’-nucleotidase deficiency, lead poisoning, dyserythropoietic disorders (inherited and acquired), myelofibrosis (possibly due to chromosome 8 duplication), or riboflavin supplementation. Consistently elevated glutathione levels have been reported in a family with mild hemolytic anemia of uncertain cause (1); however, whether this was causative or incidental was not determined.

Day(s) Performed

Monday through Friday

Report Available

10 to 13 days

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

82978

LOINC Code Information

Test ID Test Order Name Order LOINC Value
GSH Glutathione, B 2383-8

 

Result ID Test Result Name Result LOINC Value
608409 Glutathione, B 2383-8

Forms

If not ordering electronically, complete, print, and send a Benign Hematology Test Request (T755) with the specimen.