Test Code GSH Glutathione, Blood
Reporting Name
Glutathione, BPerforming Laboratory
Mayo Clinic Laboratories in RochesterUseful For
Evaluation of neonatal hyperbilirubinemia, favism or chronic or episodic hemolysis or jaundice
Evaluation for gamma-glutamylcysteine synthetase deficiency
Evaluation for glutathione synthetase deficiency causing hemolytic anemia
Evaluation for generalized glutathione synthetase deficiency with 5-oxoprolinuria
Method Name
Kinetic Spectrophotometry (KS)
Specimen Required
Container/Tube:
Preferred: Yellow top (ACD solution B)
Specimen Volume: 6 mL
Collection Instructions: Send whole blood specimen in original tube. Do not transfer blood to other containers.
Specimen Type
Whole Blood ACD-BSpecimen Minimum Volume
1 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Whole Blood ACD-B | Refrigerated | 20 days |
Reject Due To
Gross hemolysis | Reject |
Reference Values
≥12 months: 46.9-90.1 mg/dL RBC
Reference values have not been established for patients who are younger12 months of age.
Interpretation
Measurement of reduced glutathione (GSH) is used as a surrogate for the activity of the enzymes that contribute to normal levels of GSH within the red blood cell. GSH is associated with less than 25% of mean normal in individuals with deficiencies of gamma-glutamyl cysteine synthetase or glutathione synthetase.
Elevated concentrations of GSH are of uncertain significance. This finding can be nonspecific and is seen in normal neonates, pyrimidine-5’-nucleotidase deficiency, lead poisoning, dyserythropoietic disorders (inherited and acquired), myelofibrosis (possibly due to chromosome 8 duplication), or riboflavin supplementation. Consistently elevated glutathione levels have been reported in a family with mild hemolytic anemia of uncertain cause (1); however, whether this was causative or incidental was not determined.
Day(s) Performed
Monday through Friday
Report Available
10 to 13 daysTest Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
82978
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
GSH | Glutathione, B | 2383-8 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
608409 | Glutathione, B | 2383-8 |
Forms
If not ordering electronically, complete, print, and send a Benign Hematology Test Request (T755) with the specimen.