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HelpTest Code HPS12 HIV-1/HIV-2 RNA Detection Prenatal, Serum
Ordering Guidance
This test is indicated for qualitative detection and differentiation of HIV-1 and HIV-2 infection in pregnant individuals with indeterminate or inconclusive HIV serologic test results or who are suspected to be in the acute / early HIV infection stage (ie, "window period"). If serologic testing has not yet been performed on the patient, order either HVPRS / HIV Antigen and Antibody Prenatal Routine Screen, Serum or HVPPS / HIV-1 and HIV-2 Antibody Confirmation and Differentiation Prenatal, Serum, where this test will automatically be added on depending on the results of the serologic test.
Shipping Instructions
1. Ship specimen frozen on dry ice.
2. If shipment will be delayed for more than 24 hours, freeze serum specimen at -20 to -80° C until shipment on dry ice.
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Serum gel
Submission Container/Tube: Plastic vial
Specimen Volume: 1.5 mL
Collection Instructions:
1. Within 2 hours of collection, centrifuge and aliquot serum into plastic vial. .
2. Freeze aliquoted serum for shipment.
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.
Useful For
Diagnosis of HIV-1 and/or HIV-2 infection in pregnant individuals with indeterminate or inconclusive HIV serologic test results
Diagnosis of HIV-1 and/or HIV-2 infection in pregnant individuals in the acute or early phase of HIV-1 and/or HIV-2 infection
Testing Algorithm
For more information, see HIV Prenatal Testing Algorithm, Including Follow-up of Reactive Rapid Serologic Test Results.
Special Instructions
Method Name
Real-Time Reverse Transcription-Polymerase Chain Reaction (RT-PCR)
Reporting Name
HIV-1/HIV-2 RNA Detect Prenatal, SSpecimen Type
Serum SSTSpecimen Minimum Volume
0.8 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum SST | Frozen (preferred) | 40 days | |
| Refrigerated | 6 days |
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Reference Values
Undetected
Interpretation
A "Detected" result indicates that the presence of RNA of the specific virus in the plasma specimen tested, consistent with the presence of this viral infection. For example, a "Detected" result for HIV-1 RNA by this assay is indicative of HIV-1 infection in the tested individual. A follow-up plasma specimen should be collected from this individual to both verify the diagnosis and quantify the HIV RNA prior to initiation of antiviral therapy.
An "Undetected" result indicates that the assay was unable to detect RNA of the specific virus in the plasma specimen tested.
An "Inconclusive" result indicates that the presence or absence of viral RNA could not be determined with certainty after repeat testing in the laboratory, possibly due to presence of inhibitory substances in the plasma specimen tested. Collection of a new plasma for testing is recommended.
Day(s) Performed
Monday through Saturday
Report Available
1 to 3 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
87535
87538
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| HPS12 | HIV-1/HIV-2 RNA Detect Prenatal, S | 96557-4 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 616346 | HIV-1 RNA | 25835-0 |
| 616347 | HIV-2 RNA | 69353-1 |