Home
HelpTest Code JAK2V JAK2 V617F Mutation Detection, Varies
Reporting Name
JAK2 V617F Mutation Detection, VPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Useful For
Aiding in the distinction between a reactive blood cytosis and a chronic myeloproliferative disorder using extracted DNA specimens
Method Name
Quantitative Polymerase Chain Reaction (PCR)
Specimen Required
Specimen Type: Extracted DNA from blood or bone marrow
Container/Tube: 1.5- to 2-mL tube with indication of volume and concentration of the DNA
Specimen Volume: Entire specimen
Collection Instructions: Label specimen as extracted DNA from blood or bone marrow and indicate volume and concentration of the DNA.
Specimen Stability Information: Refrigerated/Ambient
Specimen Type
VariesSpecimen Minimum Volume
50 microliter at a concentration of 20 ng/microliter
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Varies | ||
Reject Due To
| Bone marrow biopsies Slides Paraffin shavings Frozen tissues and paraffin-embedded tissues Paraffin-embedded bone marrow aspirates Moderately to severely clotted |
Reject |
Reference Values
An interpretive report will be provided.
Interpretation
The results will be reported as 1 of the 2 states:
-Negative for JAK2 V617F mutation
-Positive for JAK2 V617F mutation
Positive mutation status is highly suggestive of a myeloid neoplasm but must be correlated with clinical and other laboratory features for a definitive diagnosis.
Negative mutation status does not exclude the presence of a myeloproliferative neoplasm or other neoplasm.
Results below the laboratory cutoff for positivity are of unclear clinical significance at this time.
Day(s) Performed
Monday through Saturday
Report Available
2 to 5 daysTest Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
81270-JAK2 (Janus kinase 2) (eg, myeloproliferative disorder) gene analysis, p.Val617Phe (V617F) variant
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| JAK2V | JAK2 V617F Mutation Detection, V | 43399-5 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 39724 | JAK2 Result | 53761-3 |
| 31160 | JAK2 V617F Mutation Detection, V | 43399-5 |
Forms
1. Hematopathology Patient Information (T676)
2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.