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Test Code MCRPL Macroprolactin, Serum

Reporting Name

Macroprolactin, S

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Determining biologically active levels of prolactin, in asymptomatic patients with elevated prolactin levels

 

Ruling out the presence of macroprolactin

Method Name

TOPRL: Electrochemiluminescent Immunoassay

PEGPR: Polyethylene Glycol (PEG) Precipitation Followed by Electrochemiluminescent Immunoassay


Specimen Required


Patient Preparation: For 12 hours before specimen collection do not take multivitamins or dietary supplements containing biotin (vitamin B7), which is commonly found in hair, skin, and nail supplements and multivitamins.

Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Specimen Type

Serum

Specimen Minimum Volume

0.6 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 7 days
  Frozen  90 days
  Ambient  24 hours

Reject Due To

Gross hemolysis Reject
Gross lipemia OK

Reference Values

PROLACTIN, TOTAL

Males

<18 years: not established

≥18 years: 4.0-15.2 ng/mL

Females

<18 years: not established

≥18 years: 4.8-23.3 ng/mL

 

PROLACTIN, UNPRECIPITATED

Males

<18 years: not established

≥18 years: 2.7-13.1 ng/mL

Females

<18 years: not established

≥18 years: 3.4-18.5 ng/mL

 

When the percent of the precipitated (complexed) prolactin fraction of the total prolactin is 60% or less, the result is considered negative for macroprolactin.

Interpretation

When the fraction (percentage) of polyethylene glycol (PEG)-precipitated (complexed) prolactin is 60% or less of total prolactin, the specimen is considered negative for macroprolactin. When total prolactin exceeds the upper reference limit and macroprolactin is negative, other causes for hyperprolactinemia should be explored.

 

When the fraction (percentage) of polyethylene glycol (PEG)-precipitated (complexed) prolactin is above 60%, the specimen is considered positive for the presence of macroprolactin.

 

Following polyethylene glycol (PEG)-precipitation, a patient whose unprecipitated prolactin concentration is greater than the upper limit of the unprecipitated prolactin reference interval may have hyperprolactinemia.

 

See PRL / Prolactin, Serum for interpretation of prolactin levels.

Day(s) Performed

Monday through Sunday

Report Available

Same day/1 to 3 days

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

84146 x 2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MCRPL Macroprolactin, S 78993-3

 

Result ID Test Result Name Result LOINC Value
PROU Prolactin,Unprecipitated,S 38926-2
T_PRL Prolactin,Total,S 20568-2
RATIO Prolactin, Percent PEG-precipitated 51441-4
CMT42 Interpretive Comment 48767-8

Forms

If not ordering electronically, complete, print, and send an Oncology Test Request (T729) with the specimen.

Profile Information

Test ID Reporting Name Available Separately Always Performed
TOPRL Prolactin,Total,S Yes, (order PRL) Yes
PEGPR Prolactin,Unprecipitated,S No Yes