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Test Code MMGEN Mycoplasma genitalium, Transcription-Mediated Amplification, Post-Prostatic Massage Fluid/Urine or Peritoneal Fluids


Necessary Information


Specimen source is required.



Specimen Required


Submit only 1 of the following specimens:

 

Specimen Type: Post-prostatic massage fluid/urine (VBIII)

Supplies: Aptima Urine Transport Tube (T582)

Container/Tube: Aptima Urine Specimen Transport Tube

Specimen Volume: 15 to 20 mL

Collection Instructions:

1. Patient should not have urinated for at least 1 hour prior to specimen collection.

2. Patient should void a small amount of urine prior to prostatic massage. Pre-massage urine can be discarded.

3. Patient should cease voiding and a prostatic massage should be performed by urologist or other health care professional.

4. Collect post-massage urine into a sterile, plastic, preservative-free container.

5. Transfer 2 mL of urine into the Aptima Urine Specimen Transport Tube using the disposable pipette provided within 24 hours of collection. The correct volume of urine has been added when the fluid level is between the black fill lines on the urine transport tube.

6. Place the labels on the transport tube so the black fill lines are still visible for volume confirmation at Mayo Clinic Laboratories.

7. Transport and store post-prostatic massage fluid/urine (VBIII) specimen transport container at 2 to 30° C (refrigerate is preferred temperature) within 30 days of collection. Specimen cannot be sent frozen.

 

Specimen Type: Peritoneal fluid (pelvic wash, cul-de-sac fluid)

Supplies: Aptima Thin Prep Transport Tube (T652)

Container/Tube: Aptima Specimen Transfer Tube

Specimen Volume: 1 mL

Collection Instructions:

1. Collect specimen by paracentesis using needle and syringe. Refer to the references below.

2. Thoroughly vortex specimen upon receipt to the laboratory.

3. Transfer 1 mL of specimen into the Aptima Specimen Transfer kit using a disposable transfer pipette or a pipette tip containing a filter (aerosol barrier or hydrophobic plug) within 24 hours of collection.

4. Recap the specimen transfer tube tightly.

5. Transport and store specimen container at 2 to 30° C (refrigerate is preferred temperature) within 30 days of collection. Specimen cannot be sent frozen.


Useful For

Detecting Mycoplasma genitalium in cases of suspected infection in peritoneal fluid or prostatic secretion (VBIII) fluid/urine

 

This test is not intended for use in medico-legal applications.

Method Name

Transcription-Mediated Amplification

Reporting Name

Mycoplasma genitalium, TMA, Misc

Specimen Type

Varies

Specimen Minimum Volume

Peritoneal fluid: 1 mL; Post-prostatic massage fluid/urine (VBIII): See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Varies APTIMA VIAL

Reject Due To

Overfilled or underfilled urine transport tubes
Specimen collected into a SurePath Prep device or ThinPrep vial
Midstream/clean catch urine specimen
Transport tubes contain swabs
Reject

Reference Values

Negative

Interpretation

A positive result indicates the presence of nucleic acid from Mycoplasma genitalium and strongly supports the diagnosis of a M genitalium infection.

 

A negative result indicates that nucleic acid from M genitalium was not detected in the specimen.

 

A result of inconclusive indicates that a new specimen should be collected.

 

The predictive value of an assay depends on the prevalence of the disease in a specific population. In settings with a high prevalence of sexually transmitted infections, positive assay results have a high likelihood of being truly positive. In settings with a low prevalence of sexually transmitted infections, or in any setting in which a patient's clinical signs and symptoms or risk factors are inconsistent with urogenital infection, positive results should be carefully assessed, and if appropriate, the patient retested by other methods.

Day(s) Performed

Monday through Sunday

Report Available

1 to 4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87563

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MMGEN Mycoplasma genitalium, TMA, Misc 100706-1

 

Result ID Test Result Name Result LOINC Value
MMGES Specimen Source: 31208-2
620734 Mycoplasma genitalium Result 100706-1

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.