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HelpTest Code P5NT Pyrimidine 5' Nucleotidase, Blood
Reporting Name
Pyrimidine 5' Nucleotidase, BPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Useful For
Evaluation of marked basophilic stippling
Evaluation of hemolytic anemia
Method Name
Kinetic Spectrophotometry
Specimen Required
Collection Container/Tube:
Preferred: Yellow top (ACD solution B)
Acceptable: Lavender top (EDTA)
Specimen Volume: 5 mL
Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.
Specimen Type
Whole Blood ACD-BSpecimen Minimum Volume
3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Whole Blood ACD-B | Refrigerated | 20 days |
Reject Due To
| Gross hemolysis | Reject |
Reference Values
Normal
Interpretation
A normal result indicates the absence of pyrimidine nucleotides and indicates normal pyrimidine 5' nucleotidase (P5'N) function.
An abnormal result (abnormal spectral scan) indicates the presence of pyrimidine nucleotides and possible P5'N deficiency. Enzyme activity is inhibited by heavy metal ions, including lead, mercury, copper, nickel, and cadmium. Toxic levels can cause accumulation of intracellular pyrimidine nucleotides. If results are abnormal clinical correlation is recommended to exclude heavy metal poisoning.
Day(s) Performed
Monday through Friday
Report Available
10 daysTest Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
83915
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| P5NT | Pyrimidine 5' Nucleotidase, B | 2902-5 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 2734 | Pyrimidine 5' Nucleotidase, B | 2902-5 |
Forms
If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen.