Test Code PLABN Platelet Antibody Screen, Serum
Reporting Name
Platelet Ab Screen, SPerforming Laboratory
Mayo Clinic Laboratories in RochesterUseful For
Detecting alloantibodies to epitopes on platelet glycoproteins IIb/IIIa, Ib/Ix, Ia/IIa, IV and class I human leukocyte antigens to evaluate cases of immune mediated refractoriness to platelet transfusions, posttransfusion purpura, or neonatal alloimmune thrombocytopenia
Method Name
Solid Phase Enzyme-Linked Immunosorbent Assay (ELISA)
Ordering Guidance
For neonate testing, consider sending a maternal specimen instead of a neonate specimen as unbound platelet antibodies may not be detected in the neonate serum.
This test is not recommended for the diagnosis of immune thrombocytopenia or autoimmune thrombocytopenia. Tests that are optimized to detect antibodies bound to the platelets will be useful in these situations; cell-bound platelet antibody (direct) test is strongly recommended.
Necessary Information
If ordering electronically, answer all prompt questions for timely result reporting:
1. Reason for request is required for result interpretation.
a. Use provided diagnosis options if appropriate. If specific diagnosis is unknown, select the generic answer of alloimmune thrombocytopenia.
b. Record only the diagnosis pertaining to this test.
c. Record diagnosis description instead of code.
2. Indicate if patient has had intravenous immunoglobulin (IVIg) therapy in the last month: Yes or No
Specimen Required
Patient Preparation: Do not collect within 72 hours of a platelet transfusion. Transfused platelets will interfere with this assay.
Collection Container/Tube: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Type
Serum RedSpecimen Minimum Volume
0.5 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum Red | Frozen (preferred) | 365 days | |
Refrigerated | 48 hours |
Reject Due To
Gross hemolysis | Reject |
Reference Values
Not applicable
Interpretation
This assay screens patient sera for platelet-reactive antibodies via enzyme-linked immunosorbent assay.
Day(s) Performed
Monday through Friday
Report Available
2 to 4 daysTest Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86022
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
PLABN | Platelet Ab Screen, S | 95270-5 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
PTL01 | Overall Result | 24375-8 |
PTL02 | Interpretation | 59466-3 |
PTL03 | GPIIb/IIIa (Cell-1) | 48505-2 |
PTL04 | GPIIb/IIIa (Cell-2) | 48505-2 |
PTL05 | GPIa/IIa (Cell-1) | 47084-9 |
PTL06 | GPIa/IIa (Cell-2) | 47084-9 |
PTL07 | GPIb/IX | 48506-0 |
PTL08 | GPIV | 87757-1 |
PTL09 | HLA Class I | 95269-7 |
PTL15 | Reason for request? | 29548-5 |
PTL16 | IVIg in last month? | 95268-9 |
Special Instructions
Testing Algorithm
For more information see Platelet Antibody Testing Algorithm.