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Test Code PLABN Platelet Antibody Screen, Serum

Reporting Name

Platelet Ab Screen, S

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Detecting alloantibodies to epitopes on platelet glycoproteins IIb/IIIa, Ib/Ix, Ia/IIa, IV and class I human leukocyte antigens to evaluate cases of immune mediated refractoriness to platelet transfusions, posttransfusion purpura, or neonatal alloimmune thrombocytopenia

Method Name

Solid Phase Enzyme-Linked Immunosorbent Assay (ELISA)


Ordering Guidance


For neonate testing, consider sending a maternal specimen instead of a neonate specimen as unbound platelet antibodies may not be detected in the neonate serum.

 

This test is not recommended for the diagnosis of immune thrombocytopenia or autoimmune thrombocytopenia. Tests that are optimized to detect antibodies bound to the platelets will be useful in these situations; cell-bound platelet antibody (direct) test is strongly recommended.



Necessary Information


If ordering electronically, answer all prompt questions for timely result reporting:

1. Reason for request is required for result interpretation.

 a. Use provided diagnosis options if appropriate. If specific diagnosis is unknown, select the generic answer of alloimmune thrombocytopenia.

 b. Record only the diagnosis pertaining to this test.

 c. Record diagnosis description instead of code.

2. Indicate if patient has had intravenous immunoglobulin (IVIg) therapy in the last month: Yes or No



Specimen Required


Patient Preparation: Do not collect within 72 hours of a platelet transfusion. Transfused platelets will interfere with this assay.

Collection Container/Tube: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1.5 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Specimen Type

Serum Red

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Red Frozen (preferred) 365 days
  Refrigerated  48 hours

Reject Due To

Gross hemolysis Reject

Reference Values

Not applicable

Interpretation

This assay screens patient sera for platelet-reactive antibodies via enzyme-linked immunosorbent assay.

Day(s) Performed

Monday through Friday

Report Available

2 to 4 days

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86022

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PLABN Platelet Ab Screen, S 95270-5

 

Result ID Test Result Name Result LOINC Value
PTL01 Overall Result 24375-8
PTL02 Interpretation 59466-3
PTL03 GPIIb/IIIa (Cell-1) 48505-2
PTL04 GPIIb/IIIa (Cell-2) 48505-2
PTL05 GPIa/IIa (Cell-1) 47084-9
PTL06 GPIa/IIa (Cell-2) 47084-9
PTL07 GPIb/IX 48506-0
PTL08 GPIV 87757-1
PTL09 HLA Class I 95269-7
PTL15 Reason for request? 29548-5
PTL16 IVIg in last month? 95268-9

Testing Algorithm

For more information see Platelet Antibody Testing Algorithm.