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HelpTest Code PTOX Toxoplasma gondii, Molecular Detection, PCR, Varies
Reporting Name
Toxoplasma gondii PCRPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Useful For
Supporting the diagnosis of acute cerebral, ocular, disseminated, or congenital toxoplasmosis
This test should not be used to screen healthy patients.
Method Name
Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
Necessary Information
Specimen source is required.
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Amniotic fluid
Container/Tube: Sterile container
Specimen Volume: 0.5 mL
Collection Instructions: Do not centrifuge.
Specimen Type: Spinal fluid
Supplies: Sarstedt Aliquot Tube 5 mL (T914)
Container/Tube:
Preferred: 12 x 75-mm screw cap vial
Acceptable: Sterile vial
Specimen Volume: 0.5 mL
Collection Instructions: Do not centrifuge.
Specimen Type: Fresh tissue
Supplies:M4-RT (T605)
Container/Tube:
Preferred: Multi-microbe medium (eg, M4-RT)
Acceptable: Sterile container with 1 to 2 mL of sterile saline
Specimen Volume: Entire collection
Collection Instructions: Submit only fresh tissue in a sterile container containing 1 mL to 2 mL of sterile saline or multi-microbe medium (M4-RT, M4, or M5)
Specimen Type: Ocular fluid
Supplies: Sarstedt Aliquot Tube 5 mL (T914)
Collection Container: 12 x 75-mm screw cap vial
Specimen Volume: 0.3 mL
Collection Instructions:
1. Aliquot collected fluid into screw-cap vial. Do not submit ocular fluid in syringe.
2. Do not centrifuge or dilute the specimen.
Specimen Type
VariesSpecimen Minimum Volume
Amniotic Fluid, Ocular Fluid, Spinal Fluid: 0.3 mL
Tissue: 2 × 2 mm biopsy
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Refrigerated (preferred) | 7 days | |
| Frozen | 7 days |
Reject Due To
| Heat-inactivated specimen | Reject |
Reference Values
Negative
Interpretation
A positive result indicates presence of DNA from Toxoplasma gondii.
Negative results indicate absence of detectable DNA but do not exclude the presence of organism or active or recent disease.
Day(s) Performed
Monday through Saturday
Report Available
Same day/1 to 4 daysTest Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
87798
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| PTOX | Toxoplasma gondii PCR | 29904-0 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| SRC74 | Specimen Source | 31208-2 |
| 81795 | Toxoplasma gondii PCR | 29904-0 |
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.
Testing Algorithm
For more information see Meningitis/Encephalitis Panel Algorithm