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HelpTest Code SHIGC Shigella Culture, Feces
Reporting Name
Shigella Culture, FPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Useful For
Determining whether Shigella species may be the cause of diarrhea
Reflexive testing for Shigella species from nucleic acid amplification test-positive stool
This test is generally not useful for patients hospitalized more than 3 days because the yield from specimens from these patients is very low, as is the likelihood of identifying a pathogen that has not been detected previously.
Method Name
Conventional Culture
Additional Testing Requirements
If susceptibility testing is needed, also order ZMMLS / Antimicrobial Susceptibility, Aerobic Bacteria, Varies. If susceptibility testing is not needed (eg, due to lack of recovery of Shigella species from feces), it will not be performed and the ZMMLS order will be canceled at time of report.
In some cases, local public health requirements may impact Mayo Clinic Laboratories clients, requiring, for example, submission of isolates to public health laboratories. Clients should familiarize themselves with local requirements and are responsible for submitting isolates to appropriate public health laboratories. Clients can obtain isolates of Shigella species recovered from specimens submitted to Mayo Clinic Laboratories by calling 800-533-1710 as soon as possible after reporting (to ensure viability of the bacterium).
Shipping Instructions
Specimen must arrive within 96 hours of collection.
Necessary Information
Specimen source is required.
Specimen Required
Patient Preparation: The patient should not use antacids, barium, bismuth, antidiarrheal medication, or oily laxatives before specimen collection.
Supplies: Culture and Sensitivity Stool Transport Vial (T058)
Container/Tube: Commercially available transport system specific for recovery of enteric pathogens from fecal specimens (15 mL of non-nutritive transport medium containing phenol red as a pH indicator, either Cary-Blair or Para-Pak C and S)
Specimen Volume: Representative portion of fecal specimen
Collection Instructions:
1. Collect fresh feces and submit 1 gram or 5 mL in container with transport medium.
2. Place feces in preservative within 2 hours of collection.
3. Place vial in a sealed plastic bag.
Specimen Type
FecalSpecimen Minimum Volume
1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Fecal | Ambient (preferred) | 4 days | |
| Refrigerated | 4 days |
Reject Due To
| Unpreserved feces ECOFIX preservative Formalin or PVA fixative |
Reject |
Reference Values
No growth of Shigella species.
Interpretation
The growth of Shigella species identifies a potential cause of diarrhea.
Day(s) Performed
Monday through Friday
Report Available
3 to 5 daysTest Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
87046-Shigella Culture, Stool-with isolation and preliminary examination
87077-Bacteria Identification (if appropriate)
87153-Aerobe Ident by Sequencing (if appropriate)
87077-Additional Identification Procedure (if appropriate)
87147 x 4-Serologic Agglut Method 3 Ident (if appropriate)
87077-Ident by MALDI-TOF mass spec (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| SHIGC | Shigella Culture, F | 17576-0 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| SHIGC | Shigella Culture, F | 88586-3 |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| GID | Bacteria Identification | No, (Bill Only) | No |
| ISAE | Aerobe Ident by Sequencing | No, (Bill Only) | No |
| REFID | Additional Identification Procedure | No, (Bill Only) | No |
| SHIG | Serologic Agglut Method 3 Ident | No, (Bill Only) | No |
| RMALD | Ident by MALDI-TOF mass spec | No, (Bill Only) | No |
Testing Algorithm
When this test is ordered, the reflex tests may be performed at an additional charge.
For more information see Laboratory Testing for Infectious Causes of Diarrhea