Test Code SLO Smith-Lemli-Opitz Screen, Plasma
Reporting Name
Smith-Lemli-Opitz Scrn, PPerforming Laboratory
Mayo Clinic Laboratories in RochesterUseful For
Diagnosing Smith-Lemli-Opitz syndrome (7-dehydrocholesterol reductase deficiency)
Method Name
Gas Chromatography Mass Spectrometry (GC-MS)
Necessary Information
Biochemical Genetics Patient Information (T602) is recommended, but not required, to be filled out and sent with the specimen to aid in the interpretation of test results.
Specimen Required
Collection Container/Tube:
Preferred: Green top (sodium or lithium heparin)
Acceptable: Lavender top (EDTA), pearl white top (EDTA plasma gel), yellow top (ACD solution A or B)
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions:
1. Centrifuge and aliquot plasma into plastic vial.
2. Send plasma frozen.
Specimen Type
PlasmaSpecimen Minimum Volume
0.1 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Plasma | Frozen (preferred) | 92 days | |
Refrigerated | 28 days | ||
Ambient | 14 days |
Reject Due To
Gross hemolysis | OK |
Gross lipemia | OK |
Gross icterus | OK |
Reference Values
7-DEHYDROCHOLESTEROL
≤2.0 mg/L
8-DEHYDROCHOLESTEROL
≤0.3 mg/L
Interpretation
Elevated plasma concentrations of 7-dehydrocholesterol (7-DHC) and 8-dehydrocholesterol are highly suggestive of a biochemical diagnosis of Smith-Lemli-Opitz (SLO) syndrome.
Mild elevations of these cholesterol precursors can be detected in patients with hypercholesterolemia and patients treated with some antipsychotic or antidepressant medications, including haloperidol, aripiprazole, and trazodone. However, the 7-DHC to cholesterol ratio is typically elevated only in patients with SLO syndrome.
Day(s) Performed
Tuesday, Friday
Report Available
3 to 7 daysTest Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
82542
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
SLO | Smith-Lemli-Opitz Scrn, P | 73852-6 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
29972 | Interpretation | 59462-2 |
610625 | 7-Dehydrocholesterol | 33275-9 |
610626 | 8-Dehydrocholesterol | 34671-8 |
29974 | Reviewed By | 18771-6 |
Forms
1. Biochemical Genetics Patient Information (T602)
2. If not ordering electronically, complete, print, and send a Biochemical Genetics Test Request (T798) with the specimen.