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HelpTest Code TBPZA Susceptibility, Mycobacterium tuberculosis Complex, Pyrazinamide, Varies
Infectious
Test Down Notes
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Ordering Guidance
To test for first-line agents, isoniazid, rifampin and ethambutol, order TB1LN / Antimicrobial Susceptibility, Mycobacterium tuberculosis Complex, First Line, Varies.
Isolates identified as Mycobacterium bovis and Myocobacterium bovis bacille Calmette-Guerin (BCG) will not be accepted as they are intrinsically resistant to pyrazinamide.
Additional Testing Requirements
If organism identification is not provided, CTB / Mycobacteria and Nocardia Culture, Varies or CTBID / Culture Referred for Identification, Mycobacterium and Nocardia, Varies must also be ordered and will be charged separately.
Shipping Instructions
1. See Infectious Specimen Shipping Guidelines
2. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance.
Necessary Information
Specimen source and suspected organism identification are required.
Specimen Required
Specimen Type: Organism
Supplies: Infectious Container, Large (T146)
Container/Tube: Middlebrook 7H10 agar slant
Specimen Volume: Isolate
Collection Instructions: Organism must be in pure culture, actively growing.
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.
Useful For
Susceptibility testing of Mycobacterium tuberculosis complex isolates growing in pure culture against pyrazinamide
This test is not useful for Mycobacterium bovis and Myocobacterium bovis bacille Calmette-Guerin (BCG) isolates as they are intrinsically resistant to pyrazinamide.
Additional Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| STVP | Susceptibility, Mtb Complex, PZA | No, (Bill Only) | Yes |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| MTBVP | Mtb PZA Confirmation, pnc A Sequence | No, (Bill Only) | No |
Testing Algorithm
If resistance to pyrazinamide is detected, the reflex sequencing test or confirmation of resistance will be performed at an additional charge.
If the broth microdilution test is unable to be performed (eg, due to reagent shortages, failure of the isolate to grow in the test medium), the reflex sequencing test will be performed at an additional charge.
Special Instructions
Method Name
Broth Dilution at Critical Drug Concentrations
Reporting Name
Susceptibility, Mtb Complex, PZASpecimen Type
VariesSpecimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Ambient (preferred) | ||
| Refrigerated | |||
Reject Due To
| Agar plate | Reject |
Reference Values
Results are reported as susceptible or resistant.
Interpretation
Mycobacterium tuberculosis complex isolates are reported as susceptible or resistant to pyrazinamide at the critical concentration.
Day(s) Performed
Monday through Sunday
Report Available
10 to 21 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
87188-Susceptibility, Mycobacterium tuberculosis Complex, Pyrazinamide
87153-Mtb PZA Confirmation, pncA Sequencing (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| TBPZA | Susceptibility, Mtb Complex, PZA | 56026-8 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| TBPZA | Susceptibility, Mtb Complex, PZA | 56026-8 |