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HelpTest Code URBRP Ureaplasma species, Molecular Detection, PCR, Blood
Reporting Name
Ureaplasma PCR, BPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Useful For
Rapid, sensitive, and specific identification of Ureaplasma urealyticum and Ureaplasma parvum from whole blood
This test is not intended for medicolegal use.
Method Name
Real-Time Polymerase Chain Reaction (PCR) using LightCycler and Fluorescent Resonance Energy Transfer (FRET)
Specimen Required
The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by Ureaplasma DNA is not likely.
Container/Tube:
Preferred Lavender top (EDTA)
Acceptable: Royal blue top (EDTA), pink top (EDTA), or sterile vial containing EDTA-derived aliquot
Specimen Volume: 1 mL
Collection Instructions: Send whole blood specimen in original tube (preferred
Specimen Type
Whole Blood EDTASpecimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Whole Blood EDTA | Refrigerated (preferred) | 7 days | |
| Frozen | 7 days |
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Reference Values
Not applicable
Interpretation
A positive PCR result for the presence of a specific sequence found within the Ureaplasma urealyticum and U parvum ureC gene indicates the presence of U urealyticum or U parvum DNA in the specimen.
A negative PCR result indicates the absence of detectable U urealyticum and U parvum DNA in the specimen, but does not rule-out infection as false-negative results may occur due to inhibition of PCR, sequence variability underlying the primers and probes, or the presence of U urealyticum or U parvum in quantities less than the limit of detection of the assay.
Day(s) Performed
Monday through Friday
Report Available
3 to 4 daysTest Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
87798 x 2
87999 (if appropriate for government payers)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| URBRP | Ureaplasma PCR, B | 69934-8 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| UBSRC | Specimen Source | 31208-2 |
| 44132 | Ureaplasma urealyticum PCR, B | 51988-4 |
| 44133 | Ureaplasma parvum PCR, B | 69933-0 |