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Test Code VIBC Vibrio Culture, Feces

Reporting Name

Vibrio Culture, Stool

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Determining whether Vibrio species may be the cause of diarrhea

 

This test is generally not useful for patients that have been hospitalized for more than 3 days because the yield from these patients' specimens is very low, as is the likelihood of identifying a pathogen that has not been detected previously.

Method Name

Conventional Culture


Additional Testing Requirements


1. If susceptibility testing is desired, also order ZMMLS / Antimicrobial Susceptibility, Aerobic Bacteria, Varies. If susceptibility testing is not needed (eg, due to lack of recovery of Vibrio species from feces), ZMMLS will not be performed and the order will be canceled.

2. In some cases, local public health requirements may impact Mayo Clinic Laboratories (MCL) clients, requiring submission of isolates to public health laboratories. Clients should familiarize themselves with local requirements and are responsible for submitting isolates to appropriate public health laboratories. Clients can obtain isolates of Vibrio species recovered from specimens submitted to MCL by calling 800-533-1710 as soon as possible after reporting to ensure viability of the bacterium.



Shipping Instructions


Specimen must arrive within 96 hours of collection.



Necessary Information


Specimen source is required.



Specimen Required


Patient Preparation: Medications: Do not use barium or bismuth before collection of specimen.

Supplies: Culture and Sensitivity Stool Transport Vial (T058)

Container/Tube: Commercially available transport system specific for recovery of enteric pathogens from fecal specimens (15 mL of non-nutritive transport medium containing phenol red as a pH indicator, either Cary-Blair or Para-Pak C and S)

Specimen Volume: Representative portion of fecal specimen

Collection Instructions:

1. Collect fresh feces and submit 1 gram or 5 mL in container with transport medium.

2. Place feces in preservative within 2 hours of collection.

3. Place vial in a sealed plastic bag.


Specimen Type

Fecal

Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Fecal Ambient (preferred) 4 days
  Refrigerated  4 days

Reject Due To

Fecal swab
Feces in Ecofix (green cap)
Specimen in buffered glycerol saline transport media
Reject

Reference Values

No growth of pathogen

Interpretation

The growth of Vibrio species identifies a potential cause of diarrhea.

Day(s) Performed

Monday through Sunday

Report Available

3 to 5 days

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

87046-Vibrio culture, stool

87077-Identification by MALDI-TOF mass spec (if appropriate)

87077-Additional Identification Procedure (if appropriate)

87077-Bacteria Identification (if appropriate)

87153-Aerobe Ident by Sequencing (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
VIBC Vibrio Culture, Stool 6579-7

 

Result ID Test Result Name Result LOINC Value
VIBC Vibrio Culture, Stool 6579-7

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
RMALD Ident by MALDI-TOF mass spec No, (Bill Only) No
GID Bacteria Identification No, (Bill Only) No
ISAE Aerobe Ident by Sequencing No, (Bill Only) No
REFID Additional Identification Procedure No, (Bill Only) No

Testing Algorithm

When this test is ordered, the reflex tests may be performed and charged separately.