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Test Code WNVS West Nile Virus, RNA, PCR, Molecular Detection, Serum

Reporting Name

West Nile Virus RNA, PCR, Serum

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Rapid testing for West Nile virus (WNV) RNA (lineage 1 and lineage 2) using serum specimens

 

An adjunctive test to serology for detection of early WNV infection (ie, first few days after symptom onset)

 

This test should not be used for screening asymptomatic individuals and should only be used to test patients with signs and symptoms of WNV disease.

Method Name

Real-Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)


Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container: Sterile container

Specimen Volume: 0.5 mL

Collection Instructions:

1. Within 2 hours of collection centrifuge and aliquot serum into a sterile container.

2. Serum specimens not aliquoted from the serum gel collection tube into a sterile container will be rejected.


Specimen Type

Serum

Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 7 days
  Frozen  7 days

Reject Due To

Gross hemolysis Reject
Heat-inactivated specimen Reject

Reference Values

Negative

Reference values apply to all ages.

Interpretation

A positive result indicates the presence of West Nile virus RNA and is consistent with early infection.

Day(s) Performed

Monday through Friday

Report Available

Same day/1 to 5 days

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
WNVS West Nile Virus RNA, PCR, Serum 32361-8

 

Result ID Test Result Name Result LOINC Value
608436 West Nile Virus RNA, PCR, Serum 32361-8

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Testing Algorithm

The following algorithms are available:

-Meningitis/Encephalitis Panel Algorithm

-Mosquito-borne Disease Laboratory Testing